Structure of PDB 4ch8 Chain B

Receptor sequence

>4ch8B (length=249) Species: 9606 (Homo sapiens)

IVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPW
DKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDI
ALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKEGQPS
VLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGP
FVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQF

3D structure

• PDB: 4ch8 Gpibalpha Interacts Exclusively with Exosite II of Thrombin
• Chain: B
• Resolution: 1.75 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H363 D419 E522 G523 D524 S525 G526
• Catalytic site (residue number reindexed from 1): H43 D99 E194 G195 D196 S197 G198
• Enzyme Commision number: 3.4.21.5: thrombin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	E328 I329 G330 M331 P333 W334 D433 H436 P437 C439 Y454 K455 R457 N484 K532 P534 N537 R538 W539	E8 I9 G10 M11 P13 W14 D113 H116 P117 C119 Y134 K135 R137 N156 K204 P206 N209 R210 W211
BS02	peptide	B	R418 R443 N504 F564 R565 K567 K568	R98 R123 N176 F236 R237 K239 K240
BS03	0G6	B	H363 L416 D519 A520 S525 S546 W547 G548	H43 L96 D191 A192 S197 S218 W219 G220

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0007596 blood coagulation

External links

• PDB: RCSB:4ch8, PDBe:4ch8, PDBj:4ch8
• PDBsum: 4ch8
• PubMed: 24316004
• UniProt: P00734|THRB_HUMAN Prothrombin (Gene Name=F2)