Structure of PDB 4cei Chain B

Receptor sequence

>4ceiB (length=1152) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

GAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYEL
AKTPDMGGMIRAQVFSFSRLAWRVLQHTGGMSRPFLTSTGVQMLLRKLIE
EHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMSEYRGERV
LSEKLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYV
DGFYQFTPQEFRVLEQLMVHAEHITFSLTADKPSYEREPHELELFRMTGK
TYYRLHQKAKELNLDITYKELSGTERHTKTPELAHLEAQYEARPAIPYAE
KQEALTVMQAANRRAELEGIAREIHALVREKGYRYKDVAILARQPEDYKD
MVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRYEAVFRCV
KTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSL
DDDFAQTDQEIEMENMLNDTRDWIVPPLFQLQKRMKKAKTVQEKAEALYR
YLEETDVPLKLDQERQRAEDDGRIIEAQQHQQAWDAVIQLLEEFVEMMGD
DEISLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVL
GANDGVLPARPDENGVLSDDDREWLKTIGVELSSGGRERLLDEHFLIYMA
FSSPSDRLYVSYPIADAEGKTLLPSMIVKRLEELFPHHKERLLTNEPEQV
SDEEQLMYVVNKSVAQSFTASQLRLWTREYDISDVWWSTYNVLMSEQDRL
QSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNACPFSHFA
SHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLRDEKLDWRDLTKEQCE
LFSYDAVERLAPKLQKEILLSSNRHYYVKEKLQKIVTRVSGILSEHAKAS
GFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLL
RIVAYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVL
YFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGLLLGDQEVVRLMDTTLQE
GRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGR
VSIEPYKMKNKTPCTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIK
KE

3D structure

• PDB: 4cei Structural Basis for Translocation by Addab Helicase-Nuclease and its Arrest at Chi Sites.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R1059 K1068 K1069 R1074 S1075	R1052 K1061 K1062 R1067 S1068
BS02	SF4	B	C801 S804 P1112 C1121 C1124 K1127 C1130	C794 S797 P1105 C1114 C1117 K1120 C1123
BS03	ANP	B	S10 G13 K14 T15 K16 T236 E282 R283	S9 G12 K13 T14 K15 T229 E275 R276

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003690 double-stranded DNA binding
• GO:0004386 helicase activity
• GO:0004527 exonuclease activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008409 5'-3' exonuclease activity
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0000724 double-strand break repair via homologous recombination
• GO:0006281 DNA repair
• GO:0006310 DNA recombination

External links

• PDB: RCSB:4cei, PDBe:4cei, PDBj:4cei
• PDBsum: 4cei
• PubMed: 24670664
• UniProt: P23477|ADDB_BACSU ATP-dependent helicase/deoxyribonuclease subunit B (Gene Name=addB)