Structure of PDB 4bec Chain B

Receptor sequence
>4becB (length=846) Species: 562 (Escherichia coli) [Search protein sequence]
NNFIVLDKYIKAEPTGDSYQSESDLERELIQDLRNQGYEFISVKSQSAML
ANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRKIHIDYICDF
IFDDERLENIYLIDKKNLMRNKVQIIQQFRYDVTILVNGLPLVQIELKKR
GVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKRD
ANSFDFTMNWAKSDNTLIKDLKDFTATCFQKHTLLNVLVNYSVFDSSQTL
LVMRPYQIAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFK
AARLATELDFIDKVFFVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKR
NLDKDDNKIIVTTIQKLNNLMKAESDLPVYNQQVVFIFDECHRSQFGEAQ
KNLKKKFKRYYQFGFTGTPIFPENALGSETTASVFGRELHSYVITDAIRD
EKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFLHPMRIQEITQY
ILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAANKSA
TYKPLRIATIFSFAIGEISDETFDTSAMDSSAKEFLDAAIREYNSHFKTN
FSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKN
LRYHGLMQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKN
VVLEKSYTEYMEGFTDAATGEAKRGFMTVVSELEQRFPDPTSIESEKEKK
DFVKLFGEYLRAENILQNYDEFATLKALQQIDLSDPVAVEKFKAEHYVDD
EKFAELQTIRLPADRKIQDYRSAYNDIRDWQRRWDDVVFEVDLLKS
3D structure
PDB4bec Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme.
ChainB
Resolution2.84 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.3: type I site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP B L270 V271 R273 Q276 T309 G310 G312 K313 T314 D664 R688 R691 L251 V252 R254 Q257 T290 G291 G293 K294 T295 D638 R662 R665
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0005524 ATP binding
GO:0009035 type I site-specific deoxyribonuclease activity
Biological Process
GO:0006304 DNA modification
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:4bec, PDBe:4bec, PDBj:4bec
PDBsum4bec
PubMed26039067
UniProtP10486|T1R1_ECOLX Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit (Gene Name=hsdR)

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