Structure of PDB 4a0r Chain B

Receptor sequence
>4a0rB (length=748) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
HLPLNHPTYLIWSANTSLGKTLVSTGIAASFLLQQPSSSATKLLYLKPIQ
TGFPSDSDSRFVFSKLDSLSLRRQIPISISNSVLHSSLPAAKSLGMCSLN
FRDEKTVTGAPELLCKTLYAWEAAISPHLAAERENATVEDSVVLQMIEKC
LKEEMDLLCLVETAGGVASPGPSGTLQCDLYRPFRLPGILVGDGRLGGIS
GTIAAYESLKLRGYDIAAVVFEDHGLVNEVPLTSYLRNKVPVLVLPPVPK
DPSDDLIEWFVESDGVFKALKETMVLANLERLERLNGMAKLAGEVFWWPF
TQHKLVHQETVTVIDSRCGENFSIYKASDNSSLSQQFDACASWWTQGPDP
TFQAELAREMGYTAARFGHVMFPENVYEPALKCAELLLDGVGKGWASRVY
FSDNGSTAIEIALKMAFRKFCVDHNFIVVKVIALRGSYHGDTLGAMEAQA
PSPYTGFLQQPWYTGRGLFLDPPTVFLSNGSWNISLPESFSTFTSRDEIF
DKSRDASTLARIYSAYLSKHLQAHVGALIIEPVIHGAGGMHMVDPLFQRV
LVNECRNRKIPVIFDEVFTGFWRLGVETTTELLGCKPDIACFAKLLTGGM
VPLAVTLATDAVFDSFSGDSKLKALLHGHSYSAHAMGCATAAKAIQWFKD
PETNHNITSQGKTLRELWDEELVQQISSHSAVQRVVVIGTLFALELKALY
AKSLLIMLREDGIFTRPLGNVIYLMCGPCTSPEICRRLLTKLYKRLGE
3D structure
PDB4a0r Biochemical and Structural Characterization of the Arabidopsis Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis.
ChainB
Resolution2.68 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) F326 Y473 D615 K644
Catalytic site (residue number reindexed from 1) F300 Y438 D565 K594
Enzyme Commision number 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.
6.3.3.3: dethiobiotin synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PLP B H679 S680 H629 S630
BS02 DTB B G223 I225 G197 I199
BS03 PLP B N430 G431 S432 Y473 H474 E581 D615 V617 K644 N404 G405 S406 Y438 H439 E531 D565 V567 K594
BS04 DTB B T24 Q58 T59 S195 T16 Q50 T51 S169
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0004141 dethiobiotin synthase activity
GO:0005524 ATP binding
GO:0008483 transaminase activity
GO:0016874 ligase activity
GO:0030170 pyridoxal phosphate binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0009102 biotin biosynthetic process
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4a0r, PDBe:4a0r, PDBj:4a0r
PDBsum4a0r
PubMed22547782
UniProtB0F481|BIODA_ARATH Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial (Gene Name=BIO3-BIO1)

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