Structure of PDB 4a0h Chain B

Receptor sequence
>4a0hB (length=738) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
PFHLPLNHPTYLIWSANTSLGKTLVSTGIAASFLLQQSATKLLYLKPIQT
GFPSDSDSRFVFSKLDSLSLRRQIPISISNSVLHSSLPAAKSLGGMCSLN
FRDEKTVTGAPELLCKTLYAWEAAISPHLAAERENATVEDSVVLQMIEKC
LKEEMDLLCLVETAGGVASPGPSGTLQCDLYRPFRLPGILVGDGRLGGIS
GTIAAYESLKLRGYDIAAVVFEDHGLVNEVPLTSYLRNKVPVLVLPPVPK
DPSDDLIEWFVESDGVFKALKETMVLANLERLERLNGMAKLAGEVFWWPF
TQHKLVHQETVTVIDSRCGENFSIYKASDNSSLSQQFDACASWWTQGPDP
TFQAELAREMGYTAARFGHVMFPENVYEPALKCAELLLDGVGKGWASRVY
FSDNGSTAIEIALKMAFRKFCVDHNFIVVKVIALRGSYHGDTLGAMEAQA
PSPYTGFLQQPWYTGRGLFLDPPTVFLSNGSWNISLPTFTSRDEIFDKSR
DASTLARIYSAYLSKHLQAHVGALIIEPVIHGAGGMHMVDPLFQRVLVNE
CRNRKIPVIFDEVFTGFWRLGVETTTELLGCKPDIACFAKLLTGGMVPLA
VTLATDAVFDSFSGDSKLKALLHGHSYSAHAMGCATAAKAIQWFKDPETN
HNITSQGKTLRELWDEELVQQISSHSAVQRVVVIGTLFALELSLLIMLRE
DGIFTRPLGNVIYLMCGPCTSPEICRRLLTKLYKRLGE
3D structure
PDB4a0h Biochemical and Structural Characterization of the Arabidopsis Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis.
ChainB
Resolution2.808 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) F326 Y473 D615 K644
Catalytic site (residue number reindexed from 1) F300 Y438 D561 K590
Enzyme Commision number 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.
6.3.3.3: dethiobiotin synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PLP B H679 S680 H625 S626
BS02 KAP B G678 H679 S680 G624 H625 S626
BS03 KAP B T59 S143 S195 T50 S126 S169
BS04 PLP B N430 G431 S432 H474 E581 D615 V617 K644 N404 G405 S406 H439 E527 D561 V563 K590
BS05 KAP B F326 W369 W370 K644 R775 F300 W343 W344 K590 R706
BS06 KAP B L222 G223 I225 L196 G197 I199
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0004141 dethiobiotin synthase activity
GO:0005524 ATP binding
GO:0008483 transaminase activity
GO:0016874 ligase activity
GO:0030170 pyridoxal phosphate binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0009102 biotin biosynthetic process
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4a0h, PDBe:4a0h, PDBj:4a0h
PDBsum4a0h
PubMed22547782
UniProtB0F481|BIODA_ARATH Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial (Gene Name=BIO3-BIO1)

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