Structure of PDB 3wqz Chain B

Receptor sequence

>3wqzB (length=904) Species: 224325 (Archaeoglobus fulgidus DSM 4304)

MTLDEEYLDITFLTENGFVRKRCPKCGKHFWTADPEREICGDPPCESYSF
IGNPVFKKPFELDEMREYYLNFFERRGHGRIERYPVVARWRTDIYLTIAS
IADFQPFVTSGVAPPPANPLTISQPCIRLDDLDSVGRTGRHLTLFEMMAH
HAFNYPGKEIYWKNETVAYCTELLNELGVKKEDIVYKEEPWAGGGNAGPC
LEAIVGGLEVATLVFMNLEEHPEGDIEIKGARYRKMDNYIVDTGYGLERF
VWASKGTPTVYDAIFPEVVDTIIDNSNVSFNREDERVRRIVAESSKLAGI
MGELRGERLNQLRKSVADTVGVSVEELEGIVVPLEKVYSLADHTRCILFM
LGDGLVPSNAGAGYLARLMIRRSLRLAEELELGLDLYDLVEMHKKILGFE
FDVPLSTVQEILELEKERYRTTVSKGTRLVERLVERKKKLEKDDLIELYD
SHGIPVELAVGIAAEKGAEVEMPKDIYAELAKRHSKAEKVQEKKITLQNE
YPATEKLYYDDPTLLEFEAEVIGVEGDFVILNRSAFYPESGGQDNDVGYL
IANGGKFEVVDVLEADGVVLHVVKGAKPEVGTKVKGVIDSDVRWRHMRHH
SATHVLLYSLQKVLGNHVWQAGARKEFSKARLDVTHFRRPSEEEIKEIEM
LANREILANKPIKWEWMDRIEAERKFGFRLYQGGVPPGRKIRVVQVGDDV
QACGGTHCRSTGEIGMLKILKVESIQDGVIRFEFAAGEAAIEAVEEMERL
LREASSILRVEPAKLPKTVERFFEEWKDQRKEIERLKSVIADLWADILME
RAEEFDSMKVVAEVVDADMQALQKLAERLAEKGAVGCLMAKGEGKVFVVT
FSGQKYDARELLREIGRVAKGSGGGRKDVAQGAVQQLLDREEMLDVIFRF
LSEH

3D structure

• PDB: 3wqz The selective tRNA aminoacylation mechanism based on a single G.U pair
• Chain: B
• Resolution: 3.489 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.7: alanine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	L758 F772	L758 F772
BS02	A5A	B	R128 D131 H141 L142 F145 M147 W191 E209 V210 A211 T212 D242 G244 R249	R128 D131 H141 L142 F145 M147 W191 E209 V210 A211 T212 D242 G244 R249
BS03	ZN	B	H600 H604	H600 H604

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0000166 nucleotide binding
• GO:0002161 aminoacyl-tRNA editing activity
• GO:0003676 nucleic acid binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004813 alanine-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0006419 alanyl-tRNA aminoacylation
• GO:0043039 tRNA aminoacylation
• GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3wqz, PDBe:3wqz, PDBj:3wqz
• PDBsum: 3wqz
• PubMed: 24919148
• UniProt: O28029|SYA_ARCFU Alanine--tRNA ligase (Gene Name=alaS)