Structure of PDB 3wnn Chain B

Receptor sequence

>3wnnB (length=698) Species: 1397 (Niallia circulans)

GIERVFTDKARYNPGDAVSIRVQAKNGTGSSWSGAARLEIFHLENSVYTS
SQSLSLTNGQSTTLTFTWTAPSTDFRGYFVRIDAGTLGQGATAIDVSSDF
TKYPRYGYISEFESGETALESKAKVDQLAQDYHINAWQFYDWMWRHDKMI
KRTGGSIDSTWLDLFNREISWSTLQNQIDAVHDVNGKAMAYAMIYASREN
YSPLGISPTWGIYEDSSHTNQFDVDFGDGSTYLYMFDPQNPNWQNYIHAE
YIDSINTAGFDGIHVAQMGQRSNVYDYNGNSIDLSTRFSPFLDQAKSVLS
ANNPARDNLTYNIVDGTVNGWAVNDVSKNADLDFLYSEIWYLSDSYNQLK
NYIEQLRANGGNKAVVLAAYMNYADNAGTRYEAESASMTNVSTNTNHAGY
TGSGFVDQFASTGDKVSFAINAPEAGDYSLVFRYGNNTGANSTLNLYVDG
NFVQKLYFFNQSSWGTWKHDAWYQVPLTQGAHTVELRYESGNVGAVNLDS
LTLGTFDEHSVRLADAMMSASGATHIELGDDNQMLPHEYYPNRSKTMRSS
LKNAMKDHYNFITAYENLLFDSDVVPNDTGSQFVNLTGVSASGDGSANTV
WYINKRTSDYNIVHLINLLGNDNQWRNTASQPSFQTNLPAKIYIGADETI
SDVYLASPDLSGGETQELAFTSGTDAGGKYVSFTVPELKYWNMIYMLE

3D structure

• PDB: 3wnn Structural elucidation of the cyclization mechanism of alpha-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase.
• Chain: B
• Resolution: 2.25 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.248: cycloisomaltooligosaccharide glucanotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	Y182 Y233 Q309 N354 E380 E580	Y140 Y191 Q267 N312 E338 E538
BS02	GLC	B	Y182 D183 H188 F207 Y233 Y581	Y140 D141 H146 F165 Y191 Y539
BS03	GLC	B	D512 H579 N584	D470 H537 N542
BS04	GLC	B	Q496 Y499 A513 W514 Y515	Q454 Y457 A471 W472 Y473
BS05	GLC	B	H439 Q450 W506 N539	H397 Q408 W464 N497
BS06	CA	B	E424 E426 T443 G446 D541	E382 E384 T401 G404 D499

Gene Ontology

Molecular Function

• GO:0030246 carbohydrate binding

External links

• PDB: RCSB:3wnn, PDBe:3wnn, PDBj:3wnn
• PDBsum: 3wnn
• PubMed: 24616103
• UniProt: P94286|CTA1_NIACI Cycloisomaltooligosaccharide glucanotransferase