Structure of PDB 3v8v Chain B

Receptor sequence
>3v8vB (length=636) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMW
SRLASRIMLPLGECYSDLDLYLGVQAINWTEMFNPGATFAVHRNSQYGAM
KVKDAIVDAFTRPRPNVDRDAPDIRVNVWIALDLSGDGLHLRGYRDAPIK
ETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWG
FSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTN
ARLAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYSEPALIALHSLL
GRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHV
AESEDYTNRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWV
VVQEYAHKARQRLFDIIAATISVLGIAPNKLVLKTRKGEFLEVTEYNAHL
WVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFD
LIFIDPPTFSNAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLD
GLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
3D structure
PDB3v8v Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA
ChainB
Resolution2.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.173: 23S rRNA (guanine(2445)-N(2))-methyltransferase.
2.1.1.264: 23S rRNA (guanine(2069)-N(7))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 TRV B L574 E578 R582 T587 G588 L514 E518 R522 T527 G528
BS02 GLC B L218 R220 W223 L194 R196 W199
BS03 TRV B L218 H219 L194 H195
BS04 SAM B M197 D262 S263 D290 V291 N309 Y312 M173 D238 S239 D266 V267 N285 Y288
BS05 SAM B F546 Y548 D568 M569 D597 C598 D615 S620 F486 Y488 D508 M509 D537 C538 D555 S560
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008990 rRNA (guanine-N2-)-methyltransferase activity
GO:0052915 23S rRNA (guanine(2445)-N(2))-methyltransferase activity
GO:0070043 rRNA (guanine-N7-)-methyltransferase activity
Biological Process
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0031167 rRNA methylation
GO:0032259 methylation
GO:0070475 rRNA base methylation
GO:0070476 rRNA (guanine-N7)-methylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3v8v, PDBe:3v8v, PDBj:3v8v
PDBsum3v8v
PubMed22362734
UniProtP75864|RLMKL_ECOLI Ribosomal RNA large subunit methyltransferase K/L (Gene Name=rlmL)

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