Structure of PDB 3v8d Chain B

Receptor sequence
>3v8dB (length=467) Species: 9606 (Homo sapiens) [Search protein sequence]
SRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMG
KYVHFITNPLSYHKVLCHGKYFDWKKFHFALSAKAFGHRSIDPMDGNTTE
NINDTFIKTLQGHALNSLTESMMENLQRIMRPPVAAWVTEGMYSFCYRVM
FEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFR
TAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTH
LVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLP
ICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIR
KDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKY
YYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIAKCPPLDQSRAG
LGILPPLNDIEFKYKFK
3D structure
PDB3v8d Crystal structure of human CYP7A1 in complex with 7-ketocholesterol
ChainB
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) N289 F437 C444
Catalytic site (residue number reindexed from 1) N260 F405 C412
Enzyme Commision number 1.14.14.23: cholesterol 7alpha-monooxygenase.
1.14.14.26: 24-hydroxycholesterol 7alpha-hydroxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM B H101 F129 L133 A285 S286 N289 P436 F437 T442 C444 P445 A450 I488 H78 F106 L110 A256 S257 N260 P404 F405 T410 C412 P413 A418 I453
BS02 0GV B H101 I125 R260 T278 V281 W284 S360 G485 L486 H78 I102 R231 T249 V252 W255 S328 G450 L451
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008123 cholesterol 7-alpha-monooxygenase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0033782 24-hydroxycholesterol 7alpha-hydroxylase activity
GO:0046872 metal ion binding
Biological Process
GO:0006699 bile acid biosynthetic process
GO:0006707 cholesterol catabolic process
GO:0008203 cholesterol metabolic process
GO:0014070 response to organic cyclic compound
GO:0015721 bile acid and bile salt transport
GO:0016125 sterol metabolic process
GO:0032966 negative regulation of collagen biosynthetic process
GO:0042632 cholesterol homeostasis
GO:0045471 response to ethanol
GO:0045542 positive regulation of cholesterol biosynthetic process
GO:0045717 negative regulation of fatty acid biosynthetic process
GO:0070857 regulation of bile acid biosynthetic process
GO:0071333 cellular response to glucose stimulus
GO:0071397 cellular response to cholesterol
Cellular Component
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3v8d, PDBe:3v8d, PDBj:3v8d
PDBsum3v8d
PubMed
UniProtP22680|CP7A1_HUMAN Cytochrome P450 7A1 (Gene Name=CYP7A1)

[Back to BioLiP]