Structure of PDB 3ukz Chain B

Receptor sequence

>3ukzB (length=427) Species: 10090 (Mus musculus)

QGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGL
IPKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISL
LASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLST
LACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLAD
SCWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNI
VTGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQI
QQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCG
IIEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGL
DKIEALQRHENESVYKASLNLIEKYFS

3D structure

• PDB: 3ukz Structural Basis of High-Affinity Nuclear Localization Signal Interactions with Importin-alpha
• Chain: B
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T151 A163 N361 R366 Q367
• Catalytic site (residue number reindexed from 1): T81 A93 N291 R296 Q297

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	L104 S105 R106 P110 W142 N146 S149 G150 T155 Q181 W184 N188 G191 D192 N228 W231 R238 W273 Y277 G281 N283 R315 N319 T322 E354 W357 N361 E396 W399	L34 S35 R36 P40 W72 N76 S79 G80 T85 Q111 W114 N118 G121 D122 N158 W161 R168 W203 Y207 G211 N213 R245 N249 T252 E284 W287 N291 E326 W329

Gene Ontology

Molecular Function

• GO:0061608 nuclear import signal receptor activity

Biological Process

• GO:0006606 protein import into nucleus

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3ukz, PDBe:3ukz, PDBj:3ukz
• PDBsum: 3ukz
• PubMed: 22248489
• UniProt: P52293|IMA1_MOUSE Importin subunit alpha-1 (Gene Name=Kpna2)