Structure of PDB 3ugt Chain B

Receptor sequence

>3ugtB (length=419) Species: 559292 (Saccharomyces cerevisiae S288C)

ATMTSMVSQRQDLFMTDPLSPGSMFFLPNGAKIFNKLIEFMKLQQKFKFG
FNEVVTPLIYKKTLWEKSGHWENYADDMFKVETTDEEKEEYGLKPMNCPG
HCLIFGKKDRSYNELPLRFSDFSPLHRNEASGALSGLTRLRKFHQDDGHI
FCTPSQVKSEIFNSLKLIDIVYNKIFPFVESNYFINFSTRHFIGDLKVWN
HAEQVLKEILEESGKPWKLNPGDGAFYGPKLDIMVTDHLRKTHQVATIQL
DFQLPERFDLKFKDQDNSYKRPIMIHRATFGSIERFMALLIDSNEGRWPF
WLNPYQAVIIPVNTKNVQQLDMCTALQKKLRNELEADDMEPVPLNDWHFN
VDLDIRNEPVGYRIKSAILKNYSYLIIVGDEEVQLQKYNIRERDNRKSFE
KLTMSQIWEKFIELEKNYK

3D structure

• PDB: 3ugt Yeast mitochondrial threonyl-tRNA synthetase recognizes tRNA isoacceptors by distinct mechanisms and promotes CUN codon reassignment.
• Chain: B
• Resolution: 3.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C133 R162 Q180 D182 H184 K273 H319
• Catalytic site (residue number reindexed from 1): C98 R127 Q145 D147 H149 K230 H276
• Enzyme Commision number: 6.1.1.3: threonine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	H184 H319	H149 H276

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004829 threonine-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0008270 zinc ion binding
• GO:0140101 catalytic activity, acting on a tRNA

Biological Process

• GO:0006412 translation
• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006435 threonyl-tRNA aminoacylation
• GO:0070159 mitochondrial threonyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix

External links

• PDB: RCSB:3ugt, PDBe:3ugt, PDBj:3ugt
• PDBsum: 3ugt
• PubMed: 22343532
• UniProt: P07236|SYTM_YEAST Threonine--tRNA ligase, mitochondrial (Gene Name=MST1)