Structure of PDB 3u7h Chain B

Receptor sequence
>3u7hB (length=375) Species: 10090 (Mus musculus) [Search protein sequence]
SSLGWESLKKLLVFTASGVKPQGKVAAFALDGTLITTRSGKVFPTSPSDW
RILYPEIPKKLQELAAEGYKLVIFTNQMGIGRGKLPAEVFKGKVEAVLEK
LGVPFQVLVATHAGLNRKPVSGMWDHLQEQANEGIPISVEDSVFVGDAAG
RLANWAPGRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFD
PRTISSAGPLYLPESSSLLSPNPEVVVAVGFPGAGKSTFIQEHLVSAGYV
HVNRDTLCVSSCQAALRQGKRVVIDNTNPDVPSRARYIQCAKDAGVPCRC
FNFCATIEQARHNNRFREMTDPSHAPVSDMVMFSYRKQFEPPTLAEGFLE
ILEIPFRLQHLDPALQRLYRQFSEG
3D structure
PDB3u7h Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates.
ChainB
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.1.78: polynucleotide 5'-hydroxyl-kinase.
3.1.3.32: polynucleotide 3'-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B D172 N217 Q218 M219 R258 F305 D31 N76 Q77 M78 R117 F164
BS02 MG B D172 D288 D31 D147
BS03 PO4 B L171 D172 T216 K259 L30 D31 T75 K118
Gene Ontology
Molecular Function
GO:0046403 polynucleotide 3'-phosphatase activity
GO:0046404 ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
Biological Process
GO:0006281 DNA repair

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Molecular Function
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Biological Process
External links
PDB RCSB:3u7h, PDBe:3u7h, PDBj:3u7h
PDBsum3u7h
PubMed22171004
UniProtQ9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Gene Name=Pnkp)

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