Structure of PDB 3tw6 Chain B

Receptor sequence

>3tw6B (length=1129) Species: 347834 (Rhizobium etli CFN 42)

GPISKILVANRSEIAIRVFRAANELGIKTVAIWAEEDKLALHRFKADESY
QVGRGPHLARDLGPIESYLSIDEVIRVAKLSGADAIHPGYGLLSESPEFV
DACNKAGIIFIGPKADTMRQLGNKVAARNLAISVGVPVVPATEPLAEVAK
MAAAIGYPVMLKASWGGGGRGMRVIRSEADLAKEVTEAKREAMAAFGKDE
VYLEKLVERARHVESQILGDTHGNVVHLFERDCSVQRRNQKVVERAPAPY
LSEAQRQELAAYSLKIAGATNYIGAGTVEYLMDADTGKFYFIEVNPRIQV
EHTVTEVVTGIDIVKAQIHILDGAAIGTPQSGVPNQEDIRLNGHALQCRV
TTEDPEHNFIPDYGRITAYRSASGFGIRLDGGTSYSGAIITRYYDPLLVK
VTAWAPNPLEAISRMDRALREFRIRGVATNLTFLEAIIGHPKFRDNSYTT
RFIDTTPELFQQVKRQDRATKLLTYLADVTVNGHPEAKDRPKPLENAARP
VVPYNGNGVKDGTKQLLDTLGPKKFGEWMRNEKRVLLTDTTMRDGHQSLL
ATRMRTYDIARIAGTYSHALPNLLSLECWGGATFDVSMRFLTEDPWERLA
LIREGAPNLLLQMLLRGANGVGYTNYPDNVVKYFVRQAAKGGIDLFRVFD
CLNWVENMRVSMDAIAEENKLCEAAICYTGDILNSARPKYDLKYYTNLAV
ELEKAGAHIIAVKDMAGLLKPAAAKVLFKALREATGLPIHFHTHDTSGIA
AATVLAAVEAGVDAVDAAMDALSGNTSQPCLGSIVEALSGSERDPGLDPA
WIRRISFYWEAVRNQYAAFESDLKGPASEVYLHEMPGGQFTNLKEQARSL
GLETRWHQVAQAYADANQMFGDIVKVAPSSKVVGDMALMMVSQDLTVADV
VSPDREVSFPESVVSMLKGDLGQPPSGWPEALQKKALKGEKPYTVRPGSL
LKEADLDAERKVIEKKLEREVSDFEFASYLMYPKVFTDFALASDTYGPVS
VLPTPAYFYGLADGEELFADIEKGKTLVIVNQAVSATDSQGMVTVFFELN
GQPRRIKVPDRAHGATVRRKAEPHVGAPMPGVISRVFVSSAGDVLVSIEA
MKMETAIHAEKDGEVLVKAGDQIDAKDLL

3D structure

• PDB: 3tw6 Interaction between the biotin carboxyl carrier domain and the biotin carboxylase domain in pyruvate carboxylase from Rhizobium etli.
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K124 K166 H216 R242 T281 E283 E297 N299 R301 E305 R353 D549 D655 K718 H747 H749 A882
• Catalytic site (residue number reindexed from 1): K124 K162 H212 R238 T277 E279 E293 N295 R297 E301 R349 D544 D650 K713 H742 H744 A877
• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	B	K124 K166 G171 G173 M176 E208 L210 V211 H216 Q240 N243 L285 E297 T454	K124 K162 G167 G169 M172 E204 L206 V207 H212 Q236 N239 L281 E293 T450
BS02	ZN	B	D549 K718 H747 H749	D544 K713 H742 H744
BS03	MG	B	E283 E297	E279 E293
BS04	BTI	B	R353 D399 K1119	R349 D395 K1102
BS05	MG	B	M534 R535 E537 D768	M529 R530 E532 D763

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3tw6, PDBe:3tw6, PDBj:3tw6
• PDBsum: 3tw6
• PubMed: 21958016
• UniProt: Q2K340