Structure of PDB 3tfu Chain B

Receptor sequence
>3tfuB (length=412) Species: 1773 (Mycobacterium tuberculosis) [Search protein sequence]
GLTPEQIIAVDGAHLWHPYSSISPVVAVAAHGAWLTLIRDGQPIEVLDAM
SSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKLLVDIT
PAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGYHGDT
FLAMSICDPHLAAQVFAPQVPRDYDPAYSAAFEAQLAQHAGELAAVVVEP
VVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIATGFGRTGALFAADH
AGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISAGAAGALMHGPTFMA
NPLACAVSVASVELLLGQDWRTRITELAAGLTAGLDTARALPAVTDVRVC
GAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVYAMPPYICTPAEITQ
ITSAMVEVARLV
3D structure
PDB3tfu Mechanism-based Inactivation by Aromatization of the Transaminase BioA Involved in Biotin Biosynthesis in Mycobaterium tuberculosis.
ChainB
Resolution1.94 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y25 Y157 E220 D254 A257 K283 Y407
Catalytic site (residue number reindexed from 1) Y19 Y146 E199 D233 A236 K262 Y386
Enzyme Commision number 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PL8 B G93 P317 T318 G82 P296 T297 PDBbind-CN: -logKd/Ki=3.28,Ki=520uM
BS02 PL8 B G124 S125 Y157 H158 G159 D254 I256 K283 F402 G113 S114 Y146 H147 G148 D233 I235 K262 F381 PDBbind-CN: -logKd/Ki=3.28,Ki=520uM
Gene Ontology
Molecular Function
GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0008483 transaminase activity
GO:0030170 pyridoxal phosphate binding
Biological Process
GO:0009102 biotin biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3tfu, PDBe:3tfu, PDBj:3tfu
PDBsum3tfu
PubMed21988601
UniProtP9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)

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