Structure of PDB 3tft Chain B

Receptor sequence
>3tftB (length=424) Species: 1773 (Mycobacterium tuberculosis) [Search protein sequence]
GLTPEQIIAVDGAHLWHPYSSIGRSPVVAVAAHGAWLTLIRDGQPIEVLD
AMSSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKLLVD
ITPAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGYHG
DTFLAMSICDPHGGMHSLWTDVLAAQVFAPQVPRDYDPAYSAAFEAQLAQ
HAGELAAVVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIATG
FGRTGALFAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISAGA
AGALMHGPTFMANPLACAVSVASVELLLGQDWRTRITELAAGLTAGLDTA
RALPAVTDVRVCGAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVYAM
PPYICTPAEITQITSAMVEVARLV
3D structure
PDB3tft Mechanism-based Inactivation by Aromatization of the Transaminase BioA Involved in Biotin Biosynthesis in Mycobaterium tuberculosis.
ChainB
Resolution1.95 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y25 Y157 E220 D254 A257 K283 Y407
Catalytic site (residue number reindexed from 1) Y19 Y148 E211 D245 A248 K274 Y398
Enzyme Commision number 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PLP B P317 T318 P308 T309
BS02 PLP B G124 S125 Y157 H158 G159 E220 D254 I256 K283 G115 S116 Y148 H149 G150 E211 D245 I247 K274
Gene Ontology
Molecular Function
GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0008483 transaminase activity
GO:0030170 pyridoxal phosphate binding
Biological Process
GO:0009102 biotin biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3tft, PDBe:3tft, PDBj:3tft
PDBsum3tft
PubMed21988601
UniProtP9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)

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