Structure of PDB 3sbp Chain B

Receptor sequence

>3sbpB (length=581) Species: 316 (Stutzerimonas stutzeri)

QKIHVGPGELDDYYGFWSGGHQGEVRVLGVPSMRELMRIPVFNVDSATGW
GLTNESRHIMGDSAKFLNGDCHHPHISMTDGKYDGKYLFINDKANSRVAR
IRLDIMKCDKMITVPNVQAIHGLRLQKVPHTKYVFANAEFIIPHPNDGKV
FDLQDENSYTMYNAIDAETMEMAFQVIVDGNLDNTDADYTGRFAAATCYN
SEKAFDLGGMMRNERDWVVVFDIHAVEAAVKAGDFITLGDSKTPVLDGRK
KDGKDSKFTRYVPVPKNPHGCNTSSDGKYFIAAGKLSPTCSMIAIDKLPD
LFAGKLADPRDVIVGEPELGLGPLHTTFDGRGNAYTTLFIDSQVVKWNME
EAVRAYKGEKVNYIKQKLDVHYQPGHLHASLCETNEADGKWLVALSKFSK
DRFLPVGPLHPENDQLIDISGDEMKLVHDGPTFAEPHDCIMARRDQIKTK
KIWDRNDPFFAPTVEMAKKDGINLDTDNKVIRDGNKVRVYMTSMAPAFGV
QEFTVKQGDEVTVTITNIDQIEDVSHGFVVVNHGVSMEISPQQTSSITFV
ADKPGLHWYYCSWFCHALHMEMVGRMMVEPA

3D structure

• PDB: 3sbp N2O binding at a [4Cu:2S] copper-sulphur cluster in nitrous oxide reductase.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): M551 P553
• Catalytic site (residue number reindexed from 1): M494 P496
• Enzyme Commision number: 1.7.2.4: nitrous-oxide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CUA	B	C618 W620 C622 H626 M629	C561 W563 C565 H569 M572
BS02	CUK	B	H129 H130 H178 H326 H382 H433 K454 H494	H72 H73 H121 H269 H325 H376 K397 H437
BS03	CA	B	Y256 E259 M267 D273 N324	Y199 E202 M210 D216 N267

Gene Ontology

Molecular Function

• GO:0004129 cytochrome-c oxidase activity
• GO:0005507 copper ion binding
• GO:0005509 calcium ion binding
• GO:0050304 nitrous-oxide reductase activity

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:3sbp, PDBe:3sbp, PDBj:3sbp
• PDBsum: 3sbp
• PubMed: 21841804
• UniProt: P19573|NOSZ_STUST Nitrous-oxide reductase (Gene Name=nosZ)