Structure of PDB 3s82 Chain B

Receptor sequence

>3s82B (length=376) Species: 243243 (Mycobacterium avium 104)

SEKGRLFTSESVTEGHPDKICDAISDSVLDALLAQDPRSRVAVETLVTTG
QVHVVGEVTTTAKEAFADITNTVRERILDIGYDSSDKGFDGASCGVNIGI
GAQSPGDQGLMFGYAINDTPERMPLPIALAHRLSRRLTEVRKNGVLPYLR
PDGKTQVTIEFEDDVPVRLDTVVISTQHAADIDLENTLTPDIREKVLNTV
LNDLAHDTLDTSSTRLLVNPTGKFVVGGPMGDAGLTGRKIIVDTYGGWAR
HGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERVEVQVAYAIGKAA
PVGLFIETFGTATVDPVKIEKIVPEVFDLRPGAIIRDLDLLRPIYAQTAA
YGHFGRTDVELPWEQLNKVDDLKRAI

3D structure

• PDB: 3s82 Increasing the structural coverage of tuberculosis drug targets.
• Chain: B
• Resolution: 1.73 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H17 D19 K20 E45 E58 K181 F251 D259 A260 R265 K266 K286 K290 D292
• Catalytic site (residue number reindexed from 1): H16 D18 K19 E44 E57 K154 F224 D232 A233 R238 K239 K259 K263 D265
• Enzyme Commision number: 2.5.1.6: methionine adenosyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	N394 D397 D398	N367 D370 D371

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0004478 methionine adenosyltransferase activity
• GO:0005524 ATP binding
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006556 S-adenosylmethionine biosynthetic process
• GO:0006730 one-carbon metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3s82, PDBe:3s82, PDBj:3s82
• PDBsum: 3s82
• PubMed: 25613812
• UniProt: A0QI26|METK_MYCA1 S-adenosylmethionine synthase (Gene Name=metK)