Structure of PDB 3rsz Chain B

Receptor sequence

>3rszB (length=611) Species: 4932 (Saccharomyces cerevisiae)

SRDLQNHLLFETATEVANRVGGIYSVLKSKAPITVAQYKDHYHLIGPLNK
ATYQNEVDILDWKKPEAFSDEMRPVQHALQTMESRGVHFVYGRWLIEGAP
KVILFDLDSVRGYSNEWKGDLWSLVGIPSPENDFETNDAILLGYTVAWFL
GEVAHLDSQHAIVAHFHEWLAGVALPLCRKRRIDVVTIFTTHATLLGRYL
CASGDFYNCLESVDVDHEAGRFGIYHRYCIERAAAHSADVFTTVSQITAF
EAEHLLKRKPDGILPNGLNVIKFQLHALKKEKINDFVRGHFHGCFDFDLD
NTLYFFIAGRYEYKNKGADMFIEALARLNYRLKVSGSKKTVVAFIVMPAK
NNSFTVEALKGQAEVRALENTVHEVTTSIGKRIFDHAIRYPELLKSSDKV
MLKRRILALRRPEGQLPPIVTHNMVDDANDLILNKIRQVQLFNSPSDRVK
MIFHPEFLNANNPILGLDYDEFVRGCHLGVFPSYYEPWGYTPAECTVMGV
PSITTNVSGFGSYMEDLAKDYGIYIVDRRFKAPDESVEQLVDYMEEFVKK
TAAQAINQRNRTERLSDLLDWKRMGLEYVKARQLALRRGYPDQFRELVGE
ELNDSNMDALA

3D structure

• PDB: 3rsz Multiple Glycogen-binding Sites in Eukaryotic Glycogen Synthase Are Required for High Catalytic Efficiency toward Glycogen.
• Chain: B
• Resolution: 3.009 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.11: glycogen(starch) synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	B	E117 W118 D121	E116 W117 D120
BS02	GLC	B	R86 W149 R182	R85 W148 R181
BS03	GLC	B	G152 E153	G151 E152
BS04	GLC	B	T365 H445 D450	T355 H422 D427
BS05	GLC	B	P441 I442 D450 R460	P418 I419 D427 R437

Gene Ontology

Molecular Function

• GO:0004373 alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity
• GO:0005515 protein binding
• GO:0016757 glycosyltransferase activity
• GO:0042802 identical protein binding
• GO:2001069 glycogen binding

Biological Process

• GO:0005978 glycogen biosynthetic process

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0042587 glycogen granule

External links

• PDB: RCSB:3rsz, PDBe:3rsz, PDBj:3rsz
• PDBsum: 3rsz
• PubMed: 21835915
• UniProt: P27472|GYS2_YEAST Glycogen [starch] synthase isoform 2 (Gene Name=GSY2)