Structure of PDB 3qgt Chain B

Receptor sequence

>3qgtB (length=542) Species: 5833 (Plasmodium falciparum) [Search protein sequence]

MMEQVCDVFDIYAICACCKVESKNEGKKNEVFNNYTFRGLGNKGVLPWKC
NSLDMKYFCAVTTYVNESKYEKLKYKRCKYLKLQNVVVMGRTSWESIPKK
FKPLSNRINVILSRTLKKEDFDEDVYIINKVEDLIVLLGKLNYYKCFIIG
GSVVYQEFLEKKLIKKIYFTRINSTYECDVFFPEINENEYQIISVSDVYT
SNNTTLDFIIYKKTNNDDEEEDDFVYFNFNKEKEEKNKNSIHPNDFQIYN
SLKYKYHPEYQYLNIIYDIMMNGNKQSDRTGVGVLSKFGYIMKFDLSQYF
PLLTTKKLFLRGIIEELLWFIRGETNGNTLLNKNVRIWEANGTREFLDNR
KLFHREVNDLGPIYGFQWRHFGAEYTNMYDNYENKGVDQLKNIINLIKND
PTSRRILLCAWNVKDLDQMALPPCHILCQFYVFDGKLSCIMYQRSCDLGL
GVPFNIASYSIFTHMIAQVCNLQPAQFIHVLGNAHVYNNHIDSLKIQLNR
IPYPFPTLKLNPDIKNIEDFTISDFTIQNYVHHEKISMDMAA

3D structure

PDB

3qgt Trypanosomal dihydrofolate reductase reveals natural antifolate resistance

Chain

Resolution

2.3 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	L46 D54 E382 W404 Y430 C490 R510 D513
Catalytic site (residue number reindexed from 1)	L46 D54 E316 W338 Y364 C424 R444 D447
Enzyme Commision number	1.5.1.3: dihydrofolate reductase. 2.1.1.45: thymidylate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	UMP	B	R470 R471	R404 R405
BS02	CP6	B	I14 C15 A16 D54 F58 S108 I112	I14 C15 A16 D54 F58 S93 I97
BS03	NDP	B	C15 A16 L40 G44 V45 R106 T107 S108 L127 S128 R129 T130 N144 I164 G165 G166 S167 V168 V195	C15 A16 L40 G44 V45 R91 T92 S93 L112 S113 R114 T115 N129 I149 G150 G151 S152 V153 V180
BS04	UMP	B	C490 H491 Q509 R510 S511 D513 N521 H551 Y553	C424 H425 Q443 R444 S445 D447 N455 H485 Y487

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0004146	dihydrofolate reductase activity
GO:0004799	thymidylate synthase activity
GO:0008168	methyltransferase activity
GO:0016491	oxidoreductase activity
GO:0016741	transferase activity, transferring one-carbon groups

	Biological Process
GO:0006231	dTMP biosynthetic process
GO:0006730	one-carbon metabolic process
GO:0009165	nucleotide biosynthetic process
GO:0032259	methylation
GO:0046654	tetrahydrofolate biosynthetic process

	Cellular Component
GO:0005739	mitochondrion
GO:0005829	cytosol

View graph for
Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:3qgt, PDBe:3qgt, PDBj:3qgt
PDBsum	3qgt
PubMed	21650210
UniProt	A7UD81

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