Structure of PDB 3qfr Chain B

Receptor sequence

>3qfrB (length=603) Species: 9606 (Homo sapiens)

SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEE
YDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGV
KFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF
ITWREQFWPAVCEHFGVEATGSSIRQYELVVHTDIDAAKVYMGEMGRLKS
YENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGD
HVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYR
TALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLS
WVVEARRHILAILQDCPSLRPPIDHLCELLPRLQAHYYSIASSSKVHPNS
VHICAVVVEYETKAGRINKGVATNWLRAKEPRALVPMFVRKSQFRLPFKA
TTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYL
YREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGA
HIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLD
VWS

3D structure

• PDB: 3qfr Structural basis for human NADPH-cytochrome P450 oxidoreductase deficiency.
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y459 S460 C632 D677 W679
• Catalytic site (residue number reindexed from 1): Y388 S389 C555 D600 W602
• Enzyme Commision number: 1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	H322 R427 H457 Y458 Y459 S460 C475 A476 V479 Y481 G491 V492 A493 T494 W679	H251 R356 H386 Y387 Y388 S389 C404 A405 V408 Y410 G420 V421 A422 T423 W602
BS02	FMN	B	S89 Q90 T91 T93 A94 A141 T142 Y143 G144 G177 N178 Y181 H183 F184 D211	S20 Q21 T22 T24 A25 A72 T73 Y74 G75 G108 N109 Y112 H114 F115 D142
BS03	NAP	B	R301 P536 G537 T538 C569 R570 S599 R600 K605 Y607 Q609 M638	R230 P459 G460 T461 C492 R493 S522 R523 K528 Y530 Q532 M561

Gene Ontology

Molecular Function

• GO:0003958 NADPH-hemoprotein reductase activity
• GO:0005515 protein binding
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

Biological Process

• GO:0006805 xenobiotic metabolic process
• GO:0009725 response to hormone
• GO:0022900 electron transport chain
• GO:0032770 positive regulation of monooxygenase activity
• GO:0090346 cellular organofluorine metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:3qfr, PDBe:3qfr, PDBj:3qfr
• PDBsum: 3qfr
• PubMed: 21808038
• UniProt: P16435|NCPR_HUMAN NADPH--cytochrome P450 reductase (Gene Name=POR)