Structure of PDB 3qfc Chain B

Receptor sequence

>3qfcB (length=595) Species: 9606 (Homo sapiens)

SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEE
YDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGV
KFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF
ITWREQFWLAVCEHFGVEATGEIRQYELVVHTDIDAAKVYMGEMGRLKSY
ENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDH
VAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRT
ALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSW
VVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSV
HICAVVVEYKGEATNWLRAKEPVRALVPMFVRKSQFRLPFKATTPVIMVG
PGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQF
HRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDA
RNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS

3D structure

• PDB: 3qfc Structural basis for human NADPH-cytochrome P450 oxidoreductase deficiency.
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y459 S460 C632 D677 W679
• Catalytic site (residue number reindexed from 1): Y387 S388 C547 D592 W594
• Enzyme Commision number: 1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	H322 R427 R457 Y458 Y459 S460 C475 A476 V479 G491 E492 A493 T494 W679	H250 R355 R385 Y386 Y387 S388 C403 A404 V407 G411 E412 A413 T414 W594
BS02	FMN	B	S89 T91 T93 A94 A141 T142 Y143 G144 L176 G177 N178 Y181 H183 F184 N185	S20 T22 T24 A25 A72 T73 Y74 G75 L107 G108 N109 Y112 H114 F115 N116
BS03	NAP	B	R301 G537 T538 C569 R570 S599 R600 K605 Y607 Q609 M638 S680	R229 G452 T453 C484 R485 S514 R515 K520 Y522 Q524 M553 S595

Gene Ontology

Molecular Function

• GO:0003958 NADPH-hemoprotein reductase activity
• GO:0005515 protein binding
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

Biological Process

• GO:0006805 xenobiotic metabolic process
• GO:0009725 response to hormone
• GO:0022900 electron transport chain
• GO:0032770 positive regulation of monooxygenase activity
• GO:0090346 cellular organofluorine metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:3qfc, PDBe:3qfc, PDBj:3qfc
• PDBsum: 3qfc
• PubMed: 21808038
• UniProt: P16435|NCPR_HUMAN NADPH--cytochrome P450 reductase (Gene Name=POR)