Structure of PDB 3q8p Chain B

Receptor sequence

>3q8pB (length=380) Species: 9606 (Homo sapiens)

SRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVK
KLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFD
ENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVG
SQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESC
QHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGIS
VAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLA
SLLNRVCQDGRKPHTVRLIIRRYSGRESRQCPIPSHVIQKLNYDVMTPMV
DILMKLFRNMVNVKMPFHLTLLSVCFCNLK

3D structure

• PDB: 3q8p Unique active site promotes error-free replication opposite an 8-oxo-guanine lesion by human DNA polymerase iota.
• Chain: B
• Resolution: 1.95 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	Q59 K60 Y61 E97 L99 P299 Q300 S301 S303 E304 E305 S307 R347	Q33 K34 Y35 E71 L73 P273 Q274 S275 S277 E278 E279 S281 R321
BS02	dna	B	L123 E127 K207 P240 G241 G243 K245 T246 E358 S359 R360 Q361	L97 E101 K181 P214 G215 G217 K219 T220 E327 S328 R329 Q330
BS03	DCP	B	L35 C37 T65 Y68 R71 K77 D126	L9 C11 T39 Y42 R45 K51 D100
BS04	MG	B	K237 I239 I242	K211 I213 I216
BS05	MG	B	D34 L35 D126	D8 L9 D100

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3q8p, PDBe:3q8p, PDBj:3q8p
• PDBsum: 3q8p
• PubMed: 21300901
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)