Structure of PDB 3q2j Chain B

Receptor sequence
>3q2jB (length=263) Species: 1351 (Enterococcus faecalis) [Search protein sequence]
AKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYK
GTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEE
YEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDL
ADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDG
KVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDW
EKIKYYILLDELF
3D structure
PDB3q2j Crystal structures of two aminoglycoside kinases bound with a eukaryotic protein kinase inhibitor.
ChainB
Resolution2.1501 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) K44 D190 N195 D208
Catalytic site (residue number reindexed from 1) K43 D189 N194 D207
Enzyme Commision number 2.7.1.95: kanamycin kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CA B D153 D155 E157 E262 D152 D154 E156 E261
BS02 CKI B Y42 M90 A93 L97 S194 F197 I207 Y41 M89 A92 L96 S193 F196 I206
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008910 kanamycin kinase activity
GO:0016301 kinase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
Biological Process
GO:0016310 phosphorylation
GO:0046677 response to antibiotic

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:3q2j, PDBe:3q2j, PDBj:3q2j
PDBsum3q2j
PubMed21573013
UniProtP0A3Y5|KKA3_ENTFL Aminoglycoside 3'-phosphotransferase (Gene Name=aphA)

[Back to BioLiP]