Structure of PDB 3q24 Chain B

Receptor sequence

>3q24B (length=1094) Species: 10752 (Enquatrovirus N4)

TEELKEGIDAVYPSLVGTADSKAEGIKNYFKLSFTLPEEQKSRTVGSEAP
LKDVAQALSSRARYELFTEKETANPAFNGEVIKRYKELMEHGEGIADILR
SRLAKFLNTKDVGKRFAQGTEANRWVGGKLLNIVEQDGDTFKYNEQLLQT
AVLAGLQWRLTATSNTAIKDAKDVAAITGIDQALLPEGLVEQFDTGMTLT
EAVSSLAQKIESYWGLSRNPNAPLGYTKGIPTAMAAEILAAFVESTDVVE
NIVDMSEIDPDNKKTIGLYTITELDSFDPINSFPTAIEEAVLVNPTEKMF
FGDDIPPVANTQLRNPAVRNTPEQKAALKAEQATEFYVHTPMVQFYETLG
KDRILELMGAGTLNKELLNDNHAKSLEGKNRSVEDSYNQLFSVIEQVRAQ
SEDISTVPIHYAYNMTRVGRMQMLGKYNPQSAKLVREAILPTKATLDLSN
QNNEDFSAFQLGLAQALDIKVHTMTREVMSDELTKLLEGNLKPAIDMMVE
FNTTGSLPENAVDVLNTALGDRKSFVALMALMEYSRYLVAEDKSAFVTPL
YVEADGVTNGPINAMMLMTGGLFTPDWIRNIAKGGLFIGSPNKTMNEHRS
TADNNDLYQASTNALMESLGKLRSNYASNMPIQSQIDSLLSLMDLFLPDI
NLGENGALELKRGIAKNPLTITIYGSGARGIAGKLVSSVTDAIYERMSDV
LKARAKDPNISAAMAMFGKQAASEAHAEELLARFLKDMETLTSTVPVKRK
GVLELQSTGTGAKGKINPKTYTIKGEQLKALQENMLHFFVEPLRNGITQT
VGESLVYSTEQLQKATQIQSVVLEDMFKQRVQEKLAEKAKDPTWKKGDFL
TQKELNDIQASLNNLAPMIETGSQTFYIAGSENAEVANQVLATNLDDRMR
VPMSIYAPAQAGVAGIPFMTIGTGDGMMMQTLSTMKGAPKNTLKIFDGMN
IGLNDITDASRKANEAVYTSWQGNPIKNVYESYAKFMKNVDFSKLSPEAL
EAIGKSALEYDQRENATVDDIANAASLIERNLRNIALGVDIRHKVLDKVN
LSIDQMAAVGAPYQNNGKIDLSNMTPEQQADELNKLFREELEAR

3D structure

• PDB: 3q24 X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides.
• Chain: B
• Resolution: 1.81 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	K114 R119 W129 A171 K173 D177 I181 T202 E205 K267 K268 T269 G271 R318 R421 I675 Y678 S680 G681 K849 K850 E886 A888 D901 R902 R904 V917 A918 P921	K110 R115 W125 A167 K169 D173 I177 T198 E201 K263 K264 T265 G267 R314 R417 I671 Y674 S676 G677 K845 K846 E882 A884 D897 R898 R900 V913 A914 P917
BS02	2HP	B	Q469 K474 V475 H476	Q465 K470 V471 H472
BS03	POP	B	H476 V561 T562 D610 Y612	H472 V557 T558 D606 Y608

External links

• PDB: RCSB:3q24, PDBe:3q24, PDBj:3q24
• PDBsum: 3q24
• PubMed: 21321236
• UniProt: Q859P9|RPOLV_BPN4 Virion DNA-directed RNA polymerase (Gene Name=50)