Structure of PDB 3puw Chain B

Receptor sequence
>3puwB (length=358) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
ASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKSTLLRMIAG
LETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLK
LAGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAE
PSVFLLDEPLSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLA
DKIVVLDAGRVAQVGKPLELYHYPADRFVAGFIGSPKMNFLPVTATAIDQ
VQVELPMPNRQQVWLPVENMSLGIRPEHLLPSDIADVILEGEVQVVEQLG
NETQIHIQIPSIRQNLVYRQNDVVLVEEGATFAIGLPPERCHLFREDGTA
CRRLHKEP
3D structure
PDB3puw Snapshots of the maltose transporter during ATP hydrolysis.
ChainB
Resolution2.3 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 7.5.2.1: ABC-type maltose transporter.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP B A133 S135 Q138 A132 S134 Q137
BS02 ALF B S135 G136 G137 S134 G135 G136
BS03 MG B S43 Q82 S42 Q81
BS04 ADP B V18 G39 C40 G41 K42 S43 T44 V17 G38 C39 G40 K41 S42 T43
BS05 ALF B S38 G39 K42 Q82 E159 H192 S37 G38 K41 Q81 E158 H191
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0015423 ABC-type maltose transporter activity
GO:0016887 ATP hydrolysis activity
GO:0022857 transmembrane transporter activity
GO:0140297 DNA-binding transcription factor binding
GO:0140359 ABC-type transporter activity
Biological Process
GO:0008643 carbohydrate transport
GO:0015768 maltose transport
GO:0034763 negative regulation of transmembrane transport
GO:0042956 maltodextrin transmembrane transport
GO:0055085 transmembrane transport
GO:1902344 negative regulation of maltose transport
GO:1904981 maltose transmembrane transport
Cellular Component
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0032991 protein-containing complex
GO:0043190 ATP-binding cassette (ABC) transporter complex
GO:0055052 ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
GO:1990060 maltose transport complex
GO:1990154 enzyme IIA-maltose transporter complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3puw, PDBe:3puw, PDBj:3puw
PDBsum3puw
PubMed21825153
UniProtP68187|MALK_ECOLI Maltose/maltodextrin import ATP-binding protein MalK (Gene Name=malK)

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