Structure of PDB 3pot Chain B

Receptor sequence

>3potB (length=442) Species: 145263 (Methanothermobacter marburgensis)

AKFEDKVDLYDDRGNLVEEQVPLEALSPLRNPAIKSIVQGIKRTVAVNLE
GIENALKTAKVGGPACKIMGRELDLDIVGNAESIAAAAKEMIQVTEDDDT
NVELLGGGKRALVQVPSARFDVAAEYSAAPLVTATAFVQAIINEFDVSMY
DANMVKAAVLGRYPQSVEYMGANIATMLDIPQKLEGPGYALRNIMVNHVV
AATLKNTLQAAALSTILEQTAMFEMGDAVGAFERMHLLGLAYQGMNADNL
VFDLVKANGKEGTVGSVIADLVERALEDGVIKVEKELTDYKVYGTDDLAM
WNAYAAAGLMAATMVNQGAARAAQGVSSTLLYYNDLIEFETGLPSVDFGK
VEGTAVGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPCVAAAMALDA
GTQMFSPEATSGLIKEVFSQVDEFREPLKYVVEAAAEIKNEI

3D structure

• PDB: 3pot Structural Analysis of a Ni-Methyl Species in Methyl-Coenzyme M Reductase from Methanothermobacter marburgensis.
• Chain: B
• Resolution: 1.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y367
• Catalytic site (residue number reindexed from 1): Y366
• Enzyme Commision number: 2.8.4.1: coenzyme-B sulfoethylthiotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	COM	B	F361 Y367	F360 Y366
BS02	TXZ	B	F361 F362 Y367 G369 H379	F360 F361 Y366 G368 H378
BS03	TP7	B	F361 F362 Y367 G369 H379	F360 F361 Y366 G368 H378
BS04	F43	B	S365 I366 Y367	S364 I365 Y366

Gene Ontology

Molecular Function

• GO:0016740 transferase activity
• GO:0050524 coenzyme-B sulfoethylthiotransferase activity

Biological Process

• GO:0015948 methanogenesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3pot, PDBe:3pot, PDBj:3pot
• PDBsum: 3pot
• PubMed: 21438550
• UniProt: P11560|MCRB_METTM Methyl-coenzyme M reductase I subunit beta (Gene Name=mcrB)