Structure of PDB 3pfi Chain B

Receptor sequence
>3pfiB (length=283) Species: 192222 (Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819) [Search protein sequence]
SNFDGYIGQESIKKNLNVFIAAAKKRNECLDHILFSGPAGLGKTTLANII
SYEMSANIKTTAAPMIEKSGDLAAILTNLSEGDILFIDEIHRLSPAIEEV
LYPAMEDYPKFTLIGATTRAGMLSNPLRDRFGMQFRLEFYKDSELALILQ
KAALKLNKTCEEKAALEIAKRSRSTPRIALRLLKRVRDFADVNDEEIITE
KRANEALNSLGVNELGFDAMDLRYLELLTAAKQKPIGLASIAAALSEDEN
TIEDVIEPYLLANGYIERTAKGRIASAKSYSAL
3D structure
PDB3pfi 2.7 Angstrom resolution crystal structure of a probable holliday junction DNA helicase (ruvB) from Campylobacter jejuni subsp. jejuni NCTC 11168 in complex with adenosine-5'-diphosphate
ChainB
Resolution2.695 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K65 T66 D110 T159 R218
Catalytic site (residue number reindexed from 1) K43 T44 D88 T118 R177
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP B Y28 G62 L63 G64 K65 T66 T67 Y181 I189 P217 R218 L221 Y6 G40 L41 G42 K43 T44 T45 Y140 I148 P176 R177 L180
Gene Ontology
Molecular Function
GO:0000400 four-way junction DNA binding
GO:0003677 DNA binding
GO:0005524 ATP binding
GO:0009378 four-way junction helicase activity
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0006281 DNA repair
GO:0006310 DNA recombination
GO:0032508 DNA duplex unwinding
Cellular Component
GO:0005737 cytoplasm
GO:0048476 Holliday junction resolvase complex

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Biological Process
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Cellular Component
External links
PDB RCSB:3pfi, PDBe:3pfi, PDBj:3pfi
PDBsum3pfi
PubMed
UniProtQ9PMT7|RUVB_CAMJE Holliday junction branch migration complex subunit RuvB (Gene Name=ruvB)

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