Structure of PDB 3p8o Chain B

Receptor sequence

>3p8oB (length=182) Species: 11116 (Hepatitis C virus (isolate Japanese)) [Search protein sequence]

APITAYSQQTRGLLGCIITSLTGRDKNQVDGEVQVLSTATQSFLATCVNG
VCWTVYHGAGSKTLAGPKGPITQMYTNVDQDLVGWPAPPGARSMTPCTCG
SSDLYLVTRHADVIPVRRRGDSRGSLLSPRPVSYLKGSSGGPLLCPSGHV
VGIFRAAVCTRGVAKAVDFIPVESMETTMRAS

3D structure

PDB

3p8o Combined X-ray, NMR, and kinetic analyses reveal uncommon binding characteristics of the hepatitis C virus NS3-NS4A protease inhibitor BI 201335.

Chain

Resolution

2.3 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	H57 D81 G137 S139
Catalytic site (residue number reindexed from 1)	H57 D81 G137 S139
Enzyme Commision number	2.7.7.48: RNA-directed RNA polymerase. 3.4.21.98: hepacivirin. 3.4.22.- 3.6.1.15: nucleoside-triphosphate phosphatase. 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	T4 A5 Y6 S7 Q8 T10 C16 S20 Q28 D30 G31 E32 V33 Q34 V35 L36 S37 K62 T63 L64 A65 R92	T4 A5 Y6 S7 Q8 T10 C16 S20 Q28 D30 G31 E32 V33 Q34 V35 L36 S37 K62 T63 L64 A65 R92
BS02	L5T	B	Y56 H57 V78 D81 R123 V132 L135 K136 G137 S139 F154 R155 A156 A157	Y56 H57 V78 D81 R123 V132 L135 K136 G137 S139 F154 R155 A156 A157	MOAD: Ki=1.89nM

Gene Ontology

	Molecular Function
GO:0008236	serine-type peptidase activity

	Biological Process
GO:0006508	proteolysis
GO:0019087	transformation of host cell by virus

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:3p8o, PDBe:3p8o, PDBj:3p8o
PDBsum	3p8o
PubMed	21270126
UniProt	P26662\|POLG_HCVJA Genome polyprotein

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