Structure of PDB 3orq Chain B

Receptor sequence

>3orqB (length=366) Species: 426430 (Staphylococcus aureus subsp. aureus str. Newman)

FNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHE
FIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFG
GVLINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFP
LQENEHRNQILFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFID
SNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSLPNSIELLK
PAVMMNLLGKDLDLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTND
VNQTEQDMYAKFEGSN

3D structure

• PDB: 3orq Structural and biochemical characterization of N5-carboxyaminoimidazole ribonucleotide synthetase and N5-carboxyaminoimidazole ribonucleotide mutase from Staphylococcus aureus.
• Chain: B
• Resolution: 2.23 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E254 E267 N274 S275 K348
• Catalytic site (residue number reindexed from 1): E246 E259 N266 S267 K340
• Enzyme Commision number: 6.3.4.18: 5-(carboxyamino)imidazole ribonucleotide synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	B	K184 Y185 L186 E254 E267	K176 Y177 L178 E246 E259
BS02	MG	B	E191 H214	E183 H206
BS03	POP	B	K340 R347 K348	K332 R339 K340

Gene Ontology

Molecular Function

• GO:0004638 phosphoribosylaminoimidazole carboxylase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0034028 5-(carboxyamino)imidazole ribonucleotide synthase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006164 purine nucleotide biosynthetic process
• GO:0006189 'de novo' IMP biosynthetic process

External links

• PDB: RCSB:3orq, PDBe:3orq, PDBj:3orq
• PDBsum: 3orq
• PubMed: 21795812
• UniProt: A0A0H3KFM7