Structure of PDB 3ojo Chain B

Receptor sequence

>3ojoB (length=418) Species: 1280 (Staphylococcus aureus)

SKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQNGQISIEEPG
LQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRA
LDSILPFLKKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCP
ERVLPGKILEELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTA
EMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMANKHPRVNIHQP
GPGVGGHCLAVDPYFIIAKDPENAKLIQTGREINNSMPAYVVDTTKQIIK
ALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVEL
DFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVK
SSFEDVLYYNYGNIFNFI

3D structure

• PDB: 3ojo Structure Analysis of the Staphylococcus aureus UDP-N-acetyl-mannosamine Dehydrogenase Cap5O Involved in Capsular Polysaccharide Biosynthesis.
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T119 P155 K204 N208 C258 D262
• Catalytic site (residue number reindexed from 1): T119 P155 K204 N208 C258 D262

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PDC	B	H242 R244 V245	H242 R244 V245
BS02	NAD	B	V6 G7 G9 Y10 I11 D30 I31 V80 P81 T82 T119 E151 V153	V6 G7 G9 Y10 I11 D30 I31 V80 P81 T82 T119 E151 V153
BS03	PDC	B	R152 V153 K315	R152 V153 K315
BS04	PDC	B	R152 E207 N208 R211	R152 E207 N208 R211

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
• GO:0051287 NAD binding

Biological Process

• GO:0000271 polysaccharide biosynthetic process

External links

• PDB: RCSB:3ojo, PDBe:3ojo, PDBj:3ojo
• PDBsum: 3ojo
• PubMed: 21454499
• UniProt: P95708