Structure of PDB 3o98 Chain B

Receptor sequence
>3o98B (length=603) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
APFGTLLGYAPGGVAIYSSDYSSLDDAVFRSYIDDEYMGHKWQAVEFARR
FLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAG
ALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTREL
EMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGAR
LENKGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIK
ATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITG
RMDFCMDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFNPAEGL
INELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRG
LDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIR
TGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLL
DTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKN
IYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLI
VVK
3D structure
PDB3o98 Structure and mechanism of Escherichia coli glutathionylspermidine amidase belonging to the family of cysteine; histidine-dependent amidohydrolases/peptidases
ChainB
Resolution2.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.5.1.78: glutathionylspermidine amidase.
6.3.1.8: glutathionylspermidine synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP B Y329 E330 K498 A531 K533 R538 G540 Q568 L570 W571 Q582 L603 V604 I605 Y314 E315 K483 A516 K518 R523 G525 Q553 L555 W556 Q567 L588 V589 I590
BS02 MG B E330 N332 E315 N317
BS03 MG B D318 E330 D303 E315
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008884 glutathionylspermidine amidase activity
GO:0008885 glutathionylspermidine synthase activity
GO:0016787 hydrolase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0006749 glutathione metabolic process
GO:0008216 spermidine metabolic process
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3o98, PDBe:3o98, PDBj:3o98
PDBsum3o98
PubMed21226054
UniProtP0AES0|GSP_ECOLI Bifunctional glutathionylspermidine synthetase/amidase (Gene Name=gss)

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