Structure of PDB 3nl5 Chain B

Receptor sequence
>3nl5B (length=510) Species: 5478 (Nakaseomyces glabratus) [Search protein sequence]
KFSKEQFDYSLYLVTDSGMIPEGKTLYGQVEAGLQNLVQIREKDADTKFF
IEEALQIKELCHAHNVPLIINDRIDVAMAIGADGIHVGQDDMPIPMIRKL
VGPDMVIGWSVGFPEEVDELSKMGPDMVDYIGVGTLFPMGTAGAIRVLDA
LERNNAHWCRTVGIGGLHPDNIERVLYQCVSSNGKRSLDGICVVSDIIAS
LDAAKSTKILRGLIDKTDYKFVNIGLSTKNSLTTTDEIQSIISNTLKARP
LVQHITNKVHQNFGANVTLALGSSPIMSEIQSEVNDLAAIPHATLLLNTG
SVAPPEMLKAAIRAYNDVKPIVFDPVGYSATETRLLLNNKLLTFGQFSCI
KGNSSEILGLAELSNELLIQATKIVAFKYKTVAVCTGEFDFIADGTIEGK
YSLSKGTNGTSVEDIPCVAVEAGPIEIMGDITASGCSLGSTIACMIGGQP
SEGNLFHAVVAGVMLYKAAGKIASEKCNGSGSFQVELIDALYRLTRENTP
VTWAPKLTHT
3D structure
PDB3nl5 Domain Organization in Candida glabrata THI6, a Bifunctional Enzyme Required for Thiamin Biosynthesis in Eukaryotes .
ChainB
Resolution3.3 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) R45 S114
Catalytic site (residue number reindexed from 1) R41 S110
Enzyme Commision number 2.5.1.3: thiamine phosphate synthase.
2.7.1.50: hydroxyethylthiazole kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B D340 V342 C466 D324 V326 C436
BS02 ACP B N369 T416 G417 E418 D420 I455 M458 A463 S464 G465 K497 N353 T386 G387 E388 D390 I425 M428 A433 S434 G435 K467
BS03 TZE B N272 V274 C466 N257 V259 C436
BS04 TZE B P290 I291 M292 P275 I276 M277
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004417 hydroxyethylthiazole kinase activity
GO:0004789 thiamine-phosphate diphosphorylase activity
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0046872 metal ion binding
Biological Process
GO:0009228 thiamine biosynthetic process
GO:0009229 thiamine diphosphate biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3nl5, PDBe:3nl5, PDBj:3nl5
PDBsum3nl5
PubMed20968298
UniProtQ6FV03

[Back to BioLiP]