Structure of PDB 3nak Chain B

Receptor sequence

>3nakB (length=595) Species: 9925 (Capra hircus)

SWEVGCGAPVPLVTCDEQSPYRTITGDCNNRRSPALGAANRALARWLPAE
YEDGLAVPFGWTQRKTRNGFRVPLAREVSNKIVGYLDEEGVLDQNRSLLF
MQWGQIVDHDLDFAPETELGSSEHSKVQCEEYCVQGDECFPIMFPKNDPK
LKTQGKCMPFFRAGFVCPTPPYQSLARDQINAVTSFLDASLVYGSEPSLA
SRLRNLSSPLGLMAVNQEAWDHGLAYPPFNNVKPSPCEFINTTAHVPCFQ
AGDSRASEQILLATVHTLLLREHNRLARELKRLNPHWDGEMLYQEARKIL
GAFIQIITFRDYLPIVLGSEMQKWIPPYQGYNNSVDPRISNVFTFAFRFG
HMEVPSTVSRLDENYQPWGPEAELPLHTLFFNTWRIIKDGGIDPLVRGLL
AKNSKLMNQNKMVTSELRNKLFQPTHKVHGFDLAAINLQRCRDHGMPGYN
SWRGFCGLSQPKTLKGLQAVLKNKVLAKKLLDLYKTPDNIDIWIGGNAEP
MVERGRVGPLLACLLGRQFQQIRDGDRFWWENPGVFTEKQRDSLQKVSFS
RLICDNTHITKVPLHAFQANNYPHDFVDCSAVDKLDLSPWASREN

3D structure

• PDB: 3nak Crystal structure of the complex of goat lactoperoxidase with hypothiocyanite at 3.3 A resolution
• Chain: B
• Resolution: 3.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q105 D108 H109 D110 T184 F186 D188 S190 R255 E258 H351
• Catalytic site (residue number reindexed from 1): Q105 D108 H109 D110 T184 F186 D188 S190 R255 E258 H351
• Enzyme Commision number: 1.11.1.7: peroxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	D110 T184 F186 D188 S190	D110 T184 F186 D188 S190
BS02	HEM	B	M101 G104 Q105 D108 D112 F113 A114 R255 E258 Q259 F347 R348 H351 R440	M101 G104 Q105 D108 D112 F113 A114 R255 E258 Q259 F347 R348 H351 R440
BS03	OSM	B	H109 R255 E258	H109 R255 E258

Gene Ontology

Molecular Function

• GO:0004601 peroxidase activity
• GO:0020037 heme binding

Biological Process

• GO:0006979 response to oxidative stress

External links

• PDB: RCSB:3nak, PDBe:3nak, PDBj:3nak
• PDBsum: 3nak
• UniProt: A0A452E9Y6|PERL_CAPHI Lactoperoxidase (Gene Name=LPO)