Structure of PDB 3lvm Chain B

Receptor sequence
>3lvmB (length=394) Species: 155864 (Escherichia coli O157:H7 str. EDL933) [Search protein sequence]
YGVYRAMKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKG
KHIITSKTEHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDD
TILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQ
LKVDLMSFSGHKIYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGTL
PVHQIVGMGEAYRIAKEEMATEMERLRGLRNRLWNGIKDIEEVYLNGDLE
HGAPNILNVSFNYVEGESLIMALKDLAVSSGSALEPSYVLRALGLNDELA
HSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQG
3D structure
PDB3lvm Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by IscS protein-protein interactions.
ChainB
Resolution2.05 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H104 D180 T182 Q183 K206 S323
Catalytic site (residue number reindexed from 1) H110 D186 T188 Q189 K212 S329
Enzyme Commision number 2.8.1.7: cysteine desulfurase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PLP B G74 A75 T76 H104 D180 T182 Q183 H205 K206 G80 A81 T82 H110 D186 T188 Q189 H211 K212
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0030170 pyridoxal phosphate binding
GO:0031071 cysteine desulfurase activity
GO:0046872 metal ion binding
GO:0051537 2 iron, 2 sulfur cluster binding
Biological Process
GO:0008033 tRNA processing
GO:0016226 iron-sulfur cluster assembly
GO:0044571 [2Fe-2S] cluster assembly
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3lvm, PDBe:3lvm, PDBj:3lvm
PDBsum3lvm
PubMed20404999
UniProtP0A6B9|ISCS_ECO57 Cysteine desulfurase IscS (Gene Name=iscS)

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