Structure of PDB 3kt8 Chain B

Receptor sequence

>3kt8B (length=403) Species: 4932 (Saccharomyces cerevisiae)

KEQVVTPWDVEGGVDEQGRAQNIDYDKLIKQFGTKPVNEETLKRFKQVTG
REPHHFLRKGLFFSERDFTKILDLYEQGKPFFLYTGRGPSSDSMHLGHMI
PFVFTKWLQEVFDVPLVIELTDDEKFLFKHKLTINDVKNFARENAKDIIA
VGFDPKNTFIFSDLQYMGGAFYETVVRVSRQITGSTAKAVFGFNDSDCIG
KFHFASIQIATAFPSSFPNVLGLPDKTPCLIPCAIDQDPYFRVCRDVADK
LKYSKPALLHSRFFPALQGSTTKMSASDDTTAIFMTDTPKQIQKKINKYA
FSGGQVSADLHRELGGNPDVDVAYQYLSFFKDDDVFLKECYDKYKSGELL
SGEMKKLCIETLQEFVKAFQERRAQVDEETLDKFMVPHKLVWGEKERLVA
PKP

3D structure

• PDB: 3kt8 Crystal structures of Saccharomyces cerevisiae tryptophanyl-tRNA synthetase: new insights into the mechanism of tryptophan activation and implications for anti-fungal drug design
• Chain: B
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.2: tryptophan--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	LTN	B	Y106 T107 G108 R109 E141 T143 E146 K147 Q230 C255 Q259	Y84 T85 G86 R87 E119 T121 E124 K125 Q208 C233 Q237
BS02	SO4	B	F148 T155 I156	F126 T133 I134
BS03	SO4	B	H120 S297	H98 S275

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004830 tryptophan-tRNA ligase activity
• GO:0005524 ATP binding
• GO:1990825 sequence-specific mRNA binding

Biological Process

• GO:0006412 translation
• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006436 tryptophanyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3kt8, PDBe:3kt8, PDBj:3kt8
• PDBsum: 3kt8
• PubMed: 20123733
• UniProt: Q12109|SYWC_YEAST Tryptophan--tRNA ligase, cytoplasmic (Gene Name=WRS1)