Structure of PDB 3kt3 Chain B

Receptor sequence

>3kt3B (length=405) Species: 4932 (Saccharomyces cerevisiae)

DVKEQVVTPWDVEGGVDEQGRAQNIDYDKLIKQFGTKPVNEETLKRFKQV
TGREPHHFLRKGLFFSERDFTKILDLYEQGKPFFLYTGRGPSSDSMHLGH
MIPFVFTKWLQEVFDVPLVIELTDDEKFLFKHKLTINDVKNFARENAKDI
IAVGFDPKNTFIFSDLQYMGGAFYETVVRVSRQITGSTAKAVFGFNDSDC
IGKFHFASIQIATAFPSSFPNVLGLPDKTPCLIPCAIDQDPYFRVCRDVA
DKLKYSKPALLHSRFFPALQGSTTKMSASDDTTAIFMTDTPKQIQKKINK
YAFSGGQVSADLHRELGGNPDVDVAYQYLSFFKDDDVFLKECYDKYKSGE
LLSGEMKKLCIETLQEFVKAFQERRAQVDEETLDKFMVPHKLVWGEKERL
VAPKP

3D structure

• PDB: 3kt3 Crystal structures of Saccharomyces cerevisiae tryptophanyl-tRNA synthetase: new insights into the mechanism of tryptophan activation and implications for anti-fungal drug design
• Chain: B
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.2: tryptophan--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TYM	B	Y106 T107 G108 R109 G110 G119 H120 P123 E141 T143 E146 K147 Q230 C255 A256 D258 Q259 F263 F286 K295 M296	Y86 T87 G88 R89 G90 G99 H100 P103 E121 T123 E126 K127 Q210 C235 A236 D238 Q239 F243 F266 K275 M276
BS02	SO4	B	H120 K295 S297 A298	H100 K275 S277 A278

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004830 tryptophan-tRNA ligase activity
• GO:0005524 ATP binding
• GO:1990825 sequence-specific mRNA binding

Biological Process

• GO:0006412 translation
• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006436 tryptophanyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3kt3, PDBe:3kt3, PDBj:3kt3
• PDBsum: 3kt3
• PubMed: 20123733
• UniProt: Q12109|SYWC_YEAST Tryptophan--tRNA ligase, cytoplasmic (Gene Name=WRS1)