Structure of PDB 3int Chain B

Receptor sequence

>3intB (length=374) Species: 573 (Klebsiella pneumoniae)

KSKKILIVGAGFSGAVIGRQLAEKGHQVHIIDQRDHIGGNSYDARDSETN
VMVHVYGPHIFHTDNETVWNYINKHAEMMPYVNRVKATVNGQVFSLPINL
HTINQFFSKTCSPDEARALIAEKGDEEQALRFIGKELYEAFFKGYTIKQW
GMQPSELPASILKRLPVRFNYDDNYFNHKFQGMPKCGYTQMIKSILNHEN
IKVDLQREFIVDERTHYDHVFYSGPLDAFYGYQYGRLGYRTLDFKKFIYQ
GDYQGCAVMNYCSVDVPYTRITEHKYFSPWEQHDGSVCYKEYSRACEEND
IPYYPIRQMGEMALLEKYLSLAENETNITFVGRLGTYRYLDMDVTIAEAL
KTAEVYLNSLTDNQPMPVFTVSVG

3D structure

• PDB: 3int X-ray crystallography reveals a reduced substrate complex of UDP-galactopyranose mutase poised for covalent catalysis by flavin .
• Chain: B
• Resolution: 2.51 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R174 R178 R250 R280 E301 Y349 D351
• Catalytic site (residue number reindexed from 1): R164 R168 R240 R270 E291 Y339 D341
• Enzyme Commision number: 5.4.99.9: UDP-galactopyranose mutase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FDA	B	V9 G10 G12 S14 D33 Q34 R35 G40 P59 H60 I61 F219 G234 Y314 R343 Y349 L350 D351 M352 T355	V8 G9 G11 S13 D32 Q33 R34 G39 P58 H59 I60 F209 G224 Y304 R333 Y339 L340 D341 M342 T345
BS02	GDU	B	P59 I61 F152 Y155 W160 L172 R174 L175 Y185 F186 N270 R280 Y314 Y349	P58 I60 F142 Y145 W150 L162 R164 L165 Y175 F176 N260 R270 Y304 Y339

Gene Ontology

Molecular Function

• GO:0008767 UDP-galactopyranose mutase activity

External links

• PDB: RCSB:3int, PDBe:3int, PDBj:3int
• PDBsum: 3int
• PubMed: 19719175
• UniProt: Q48485|GLF1_KLEPN UDP-galactopyranose mutase (Gene Name=rfbD)