Structure of PDB 3hgo Chain B

Receptor sequence

>3hgoB (length=363) Species: 4081 (Solanum lycopersicum)

PLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFL
ITEGTMISPTSAGYPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHV
GRASHEVYQPAGAAPISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEI
SQVVEDYRRSALNAIEAGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGS
LANRCKFITQVVQAVVSAIGADRVGVRVSPAIDYLDAMDSNPLSLGLAVV
ERLNKIQLHSGSKLAYLHVTQPRLGSEEEEARLMRTLRNAYQGTFICSGG
YTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKYNRKTFYT
QDPVVGYTDYPFL

3D structure

• PDB: 3hgo Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.
• Chain: B
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T33 H185 H188 Y190 R237 L245
• Catalytic site (residue number reindexed from 1): T23 H175 H178 Y180 R227 L235
• Enzyme Commision number: 1.3.1.42: 12-oxophytodienoate reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	P31 M32 T33 Q106 H185 R237 S319 G320 G321 G342 R343 F369 Y370	P21 M22 T23 Q96 H175 R227 S298 G299 G300 G321 R322 F348 Y349

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0016629 12-oxophytodienoate reductase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006633 fatty acid biosynthetic process
• GO:0009695 jasmonic acid biosynthetic process
• GO:0031408 oxylipin biosynthetic process

Cellular Component

• GO:0005777 peroxisome

External links

• PDB: RCSB:3hgo, PDBe:3hgo, PDBj:3hgo
• PDBsum: 3hgo
• PubMed: 19660473
• UniProt: Q9FEW9|OPR3_SOLLC 12-oxophytodienoate reductase 3 (Gene Name=OPR3)