Structure of PDB 3hfz Chain B

Receptor sequence

>3hfzB (length=785) Species: 274 (Thermus thermophilus)

MRVPFSWLKAYVPELESPEVLEERLAGLGFETDRIERVFPIPRGVVFARV
LEAHPIPGTRLKRLVLDAGRTVEVVSGAENARKGIGVALALPGTELPGLG
QKVGERVIQGVRSFGMALSPRELGVGEYGGGLLEFPEDALPPGTPLSEAW
PEEVVLDLEVTPNRPDALGLLGLARDLHALGYALVEPEAALKAEALPLPF
ALKVEDPEGAPHFTLGYAFGLRVAPSPLWMQRALFAAGMRPINNVVDVTN
YVMLERAQPMHAFDLRFVGEGIAVRRAREGERLKTLDGVERTLHPEDLVI
AGWRGEESFPLGLAGVMGGAESEVREDTEAIALEVACFDPVSIRKTARRH
GLRTEASHRFERGVDPLGQVPAQRRALSLLQALAGARVAEALLEAGSPKP
PEAIPFRPEYANRLLGTSYPEAEQIAILKRLGCRVEGEGPTYRVTPPSHR
LDLRLEEDLVEEVARIQGYETIPLALPAFFPAPDNRGVEAPYRKEQRLRE
VLSGLGFQEVYTYSFMDPEDARRFRLDPPRLLLLNPLAPEKAALRTHLFP
GLVRVLKENLDLDRPERALLFEVGRVFREREETHLAGLLFGEGVGLPWAK
ERLSGYFLLKGYLEALFARLGLAFRVEAQAFPFLHPGVSGRVLVEGEEVG
FLGALHPEIAQELELPPVHLFELRLPLPDKPLAFQDPSRHPAAFRDLAVV
VPAPTPYGEVEALVREAAGPYLESLALFDLYQGPPLPEGHKSLAFHLRFR
HPKRTLRDEEVEEAVSRVAEALRARGFGLRGLDTP

3D structure

• PDB: 3hfz Eukaryotic cytosolic and mitochondrial phenylalanyl-tRNA synthetases catalyze the charging of tRNA with the meta-tyrosine
• Chain: B
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.20: phenylalanine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MTY	B	H261 G315 M317 E334	H261 G315 M317 E334

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0000287 magnesium ion binding
• GO:0003723 RNA binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004826 phenylalanine-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0006432 phenylalanyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0009328 phenylalanine-tRNA ligase complex

External links

• PDB: RCSB:3hfz, PDBe:3hfz, PDBj:3hfz
• PDBsum: 3hfz
• PubMed: 19549855
• UniProt: Q5SGX1|SYFB_THET8 Phenylalanine--tRNA ligase beta subunit (Gene Name=pheT)