Structure of PDB 3h3j Chain B

Receptor sequence

>3h3jB (length=314) Species: 93062 (Staphylococcus aureus subsp. aureus COL)

FKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKH
ATPYSPTTVRVKAGEYSDCHDADLVVICAGARQKPGETRLDLVSKNLKIF
KSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDS
ARFRLLLSEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQ
RPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDA
VLTVSALLEGEYEEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSA
KTLKDIMAEAEELK

3D structure

• PDB: 3h3j Crystal structure of lactate dehydrogenase mutant (A85R) from staphylococcus aureus complexed with NAD and pyruvate
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R92 D152 R155 H179
• Catalytic site (residue number reindexed from 1): R89 D149 R152 H176
• Enzyme Commision number: 1.1.1.27: L-lactate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	G15 A16 V17 D38 L39 C81 A82 G83 A84 R85 A122 N124 H179 T232 V236	G12 A13 V14 D35 L36 C78 A79 G80 A81 R82 A119 N121 H176 T229 V233
BS02	PYR	B	R92 N124 L151 R155 H179 A222 T232	R89 N121 L148 R152 H176 A219 T229

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004459 L-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process

• GO:0006089 lactate metabolic process
• GO:0006090 pyruvate metabolic process
• GO:0006096 glycolytic process
• GO:0019752 carboxylic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3h3j, PDBe:3h3j, PDBj:3h3j
• PDBsum: 3h3j
• UniProt: Q5HJD7|LDH1_STAAC L-lactate dehydrogenase 1 (Gene Name=ldh1)