Structure of PDB 3gv8 Chain B

Receptor sequence

>3gv8B (length=374) Species: 9606 (Homo sapiens)

SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGV
KKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGF
DENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLV
GSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPES
CQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGI
SVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELL
ASLLNRVCRKPHTVRLIIRRYGRESRQCPIPSHVIQKDVMTPMVDILMKL
FRNMVNVKMPFHLTLLSVCFCNLK

3D structure

• PDB: 3gv8 Structural basis of error-prone replication and stalling at a thymine base by human DNA polymerase iota
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	Q59 K60 Y61 L62 L78 E97 L99 P299 Q300 S301 F302 S303 E304 E305 S307 R331 R347	Q34 K35 Y36 L37 L53 E72 L74 P274 Q275 S276 F277 S278 E279 E280 S282 R306 R319
BS02	dna	B	E127 K207 P240 G241 I242 G243 K245 T246 E358 S359	E102 K182 P215 G216 I217 G218 K220 T221 E324 S325
BS03	MG	B	D34 L35 D126	D9 L10 D101
BS04	DGT	B	D34 L35 D36 C37 F38 Y39 T65 Y68 R71 K77 D126	D9 L10 D11 C12 F13 Y14 T40 Y43 R46 K52 D101

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3gv8, PDBe:3gv8, PDBj:3gv8
• PDBsum: 3gv8
• PubMed: 19440206
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)