Structure of PDB 3gv5 Chain B

Receptor sequence

>3gv5B (length=379) Species: 9606 (Homo sapiens)

RVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKK
LMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDE
NFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGS
QIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQ
HLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISV
AQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLAS
LLNRVCQDGRKPHTVRLIIRRYSGRESRQCPIPSHVIQKLNYDVMTPMVD
ILMKLFRNMVNVKMPFHLTLLSVCFCNLK

3D structure

• PDB: 3gv5 Structural basis of error-prone replication and stalling at a thymine base by human DNA polymerase iota
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	D126 E127 K207 G241 G243 K245 T246 E358 S359 R360 Q361	D99 E100 K180 G214 G216 K218 T219 E326 S327 R328 Q329
BS02	dna	B	Q59 K60 Y61 L62 E97 L99 R103 K237 Y244 P299 Q300 S301 F302 S303 E305 S307 R347 R357	Q32 K33 Y34 L35 E70 L72 R76 K210 Y217 P272 Q273 S274 F275 S276 E278 S280 R320 R325
BS03	CA	B	S296 Q300	S269 Q273
BS04	CA	B	D34 L35 D126	D7 L8 D99
BS05	ADI	B	D36 C37 F38 T65 R71 K77	D9 C10 F11 T38 R44 K50

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3gv5, PDBe:3gv5, PDBj:3gv5
• PDBsum: 3gv5
• PubMed: 19440206
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)