Structure of PDB 3gfz Chain B

Receptor sequence

>3gfzB (length=394) Species: 272620 (Klebsiella pneumoniae subsp. pneumoniae MGH 78578)

MLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQV
LEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDLRLF
ETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPLQPSRW
MMMAPQHLPGQPCQFALQAIVEPAKKRVSSFEALIRSPTGGSPVEMFAAI
AAEDRYRFDLESKAYAFALAGQLPLGKHQLAINLLPGSLYHHPDAVGWLM
DSLLAAGLRPDQVLIEVTETEVITCFDQFRKVLKALRVAGMKLAIDDFGA
GYSGLSLLTRFQPDKIKVDAELVRDIHISGTKQAIVASVVRCCEDLGITV
VAEGVETLEEWCWLQSVGIRLFQGFLFSRPCLNGIGEICWPVAR

3D structure

• PDB: 3gfz Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase.
• Chain: B
• Resolution: 2.05 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	C2E	B	Q174 I191 R192 P199 D215 K219 N239 D302 G360 E362 G380 F381	Q168 I185 R186 P193 D209 K213 N233 D296 G354 E356 G374 F375
BS02	MN	B	E188 N239 E272 D302	E182 N233 E266 D296
BS03	MN	B	D302 D303 E359	D296 D297 E353
BS04	FMN	B	Y7 L23 A27 K30 N31 F47 Q49 L61 I65 D68 R70 H71	Y7 L23 A27 K30 N31 F47 Q49 L61 I65 D68 R70 H71

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0009882 blue light photoreceptor activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding
• GO:0071949 FAD binding

Biological Process

• GO:0009785 blue light signaling pathway

External links

• PDB: RCSB:3gfz, PDBe:3gfz, PDBj:3gfz
• PDBsum: 3gfz
• PubMed: 19536266
• UniProt: A6T8V8