Structure of PDB 3fuc Chain B

Receptor sequence

>3fucB (length=274) Species: 9913 (Bos taurus)

NGYTYEDYQDTAKWLLSHTEQRPQVAVICGSGLGGLVNKLTQAQTFDYSE
IPNFPGRLVFGILNGRACVMMQGRFHMYEGYPFWKVTFPVRVFRLLGVET
LVVTNAAGGLNPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFP
AMSDAYDRDMRQKAHSTWKQMGEQRELQEGTYVMLGGPNFETVAECRLLR
NLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESQGKANHEEV
LEAGKQAAQKLEQFVSLLMASIPV

3D structure

• PDB: 3fuc 1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue inhibitor bearing one feature of the postulated transition state.
• Chain: B
• Resolution: 1.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S33 H86 Y88 E89 A116 M219 S220 N243 V245 H257
• Catalytic site (residue number reindexed from 1): S31 H76 Y78 E79 A106 M209 S210 N233 V235 H247
• Enzyme Commision number: 2.4.2.1: purine-nucleoside phosphorylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	9D9	B	S33 R84 H86 N115 A116 A117 G118 F200 E201 V217 M219 S220 T242 N243	S31 R74 H76 N105 A106 A107 G108 F190 E191 V207 M209 S210 T232 N233	MOAD: Kd=190pM PDBbind-CN: -logKd/Ki=9.72,Kd=190pM BindingDB: IC50=10.2nM,Ki=1nM,Kd=0.1nM

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004731 purine-nucleoside phosphorylase activity
• GO:0016757 glycosyltransferase activity
• GO:0016763 pentosyltransferase activity
• GO:0047975 guanosine phosphorylase activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006166 purine ribonucleoside salvage
• GO:0009116 nucleoside metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3fuc, PDBe:3fuc, PDBj:3fuc
• PDBsum: 3fuc
• PubMed: 19944078
• UniProt: P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Gene Name=PNP)