Structure of PDB 3f73 Chain B

Receptor sequence

>3f73B (length=670) Species: 262724 (Thermus thermophilus HB27) [Search protein sequence]

LGKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGREEVYPLLAQVARRA
GGVTVRMGDGLASWSPPEVLVLEGTLARMGQTYAYRLYPKGRRPLDPKDP
GERSVLSALARRLLQERLRRLEGVWVEGLAVYRREHARGPGWRVLGGAVL
DLWVSDSGAFLLEVDPAYRILCEMSLEAWLAQGHPLPKRVRNAYDRRTWE
LLRLGEEDPKELPLPGGLSLLDYHASKGRLQGREGGRVAWVADPKDPRKP
IPHLTGLLVPVLTLEDLHGSLALSLPWEERRRRTREIASWIGRRLGLGTP
EAVRAQAYRLSIPKLMGRRAVSKPADALRVGFYRAQETALALLRLDGAQG
WPEFLRRALLRAFGASGASLRLHTLHAHPSQGLAFREALRKAKEEGVQAV
LVLTPPMAWEDRNRLKALLLREGLPSQILNVPLREEERHRWENALLGLLA
KAGLQVVALSGYPAELAVGFDAGGRESFRFGGAACAVGGGHLLWTLPEAQ
AIPQEVVWDLLEETLWAFRRAGRLPSRVLLLRDGRVPQDEFALALEALAR
EGIAYDLVSVRKSGGGRVYPVQGRLADGLYVPLEDKTFLLLTVHRRGTPR
PLKLVHEAGDTPLEALAHQIFHLTRLYPASGFAFPRLPAPLHLADRLVKE
VGRLGIRHLKEVDREKLFFV

3D structure

PDB

3f73 Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex.

Chain

Resolution

3.0 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	Q48 R52 A80 R81 M82 A170 Y171 R172 I173 R192 Y197 L217 Y226 H227 R232 I254 P255 V264 L279 S280 R286 M413 W415 R418 K422 Q433 I434 L435 N436 H445 R446 N449 R580 T613 P614 R615 A644 P650 R651 H657	Q45 R49 A77 R78 M79 A167 Y168 R169 I170 R189 Y194 L214 Y223 H224 R229 I251 P252 V261 L273 S274 R280 M407 W409 R412 K416 Q427 I428 L429 N430 H439 R440 N443 R567 T598 P599 R600 A629 P635 R636 H642
BS02	rna	B	L267 K329 F647	L264 K323 F632

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004520	DNA endonuclease activity
GO:0030145	manganese ion binding
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0044355	clearance of foreign intracellular DNA

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Molecular Function

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Biological Process

External links

PDB	RCSB:3f73, PDBe:3f73, PDBj:3f73
PDBsum	3f73
PubMed	19092929
UniProt	Q746M7\|AGO_THET2 Protein argonaute (Gene Name=ago)

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