Structure of PDB 3f5m Chain B

Receptor sequence
>3f5mB (length=462) Species: 5691 (Trypanosoma brucei) [Search protein sequence]
RVTSKLVKAHRAMLNSVTQEDLKVDRLPGADYPNPSKKDKTDYIMYNPRP
RDSSENPVSVSPLLCELAAARSRIHFNPTETTIGIVTCGGICPGLNDVIR
SITLTGINVYNVKRVIGFRFGYWGLSKKGSQTAIELHRGRVTNIHHYGGT
ILGSSRGPQDPKEMVDTLERLGVNILFTVGGDGTQRGALVISQEAKRRGV
DISVFGVPKTIDNDLSFSHRTFGFQTAVEKAVQAIRAAYAEAVSANYGVG
VVKLMGRDSGFIAAQAAVASAQANICLVPENPISEQEVMSLLERRFCHSR
SCVIIVAEGFGQDWGRYDASGNKKLIDIGVILTEKVKAFLKANKSRYPDS
TVKYIDPSYMIRACPPSANDALFCATLATLAVHEAMAGATGCIIAMRHNN
YILVPIKVATSVRRVLDLRGQLWRQVREITVDLGSDVRLARKLEIRRELE
AINRNRDRLHEE
3D structure
PDB3f5m The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases.
ChainB
Resolution2.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G107 R173 G174 D199 G200 K226 T227 D229 D231 R274
Catalytic site (residue number reindexed from 1) G90 R156 G157 D182 G183 K209 T210 D212 D214 R257
Enzyme Commision number 2.7.1.11: 6-phosphofructokinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP B Y139 R173 G174 P175 G198 G200 T201 R203 G204 S341 N343 Y122 R156 G157 P158 G181 G183 T184 R186 G187 S320 N322
Gene Ontology
Molecular Function
GO:0003872 6-phosphofructokinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0042301 phosphate ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006002 fructose 6-phosphate metabolic process
GO:0006096 glycolytic process
GO:0016310 phosphorylation
GO:0061615 glycolytic process through fructose-6-phosphate
Cellular Component
GO:0005737 cytoplasm
GO:0020015 glycosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3f5m, PDBe:3f5m, PDBj:3f5m
PDBsum3f5m
PubMed19084537
UniProtO15648|PFKA_TRYBB ATP-dependent 6-phosphofructokinase (Gene Name=pfk)

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