Structure of PDB 3dw9 Chain B

Receptor sequence

>3dw9B (length=334) Species: 1911 (Streptomyces griseus)

PFTYSIEATRNLATTERCIQDIRNAPVRNRSTQFQLAQQNMLAYTFGEVI
PGFASAGINGMDYRDVIGRPVENAVTEGTHFFRDDFRVDSNAKAKVAGDI
FEIVSSAVMWNCAARWNSLMVGEGWRSQPRYSRPTLSPSPRRQVAVLNLP
RSFDWVSLLVPESQEVIEEFRAGLRKDGLGLPTSTPDLAVVVLPEEFQND
EMWREEIAGLTRPNQILLSGAYQRLQGRVQPGEISLAVAFKRSLRSDRLY
QPLYEANVMQLLLEGKLGAPKVEFEVHTLAPEGTNAFVTYEAASLYGLAE
VHRAIRELYVPPTAADLARRFFAFLNERMELVNG

3D structure

• PDB: 3dw9 The structure of SgrAI bound to DNA; recognition of an 8 base pair target.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	S91 A95 K96 G99 D100 R152 S153 D188 K242 R243 S244 R246 R249	S90 A94 K95 G98 D99 R151 S152 D187 K241 R242 S243 R245 R248
BS02	dna	B	R31 T33 Q36 L37 Q39 Q40 D90 N92 A93 K96 V97 R246 S247 D248 G284	R30 T32 Q35 L36 Q38 Q39 D89 N91 A92 K95 V96 R245 S246 D247 G283
BS03	MN	B	D188 F241	D187 F240
BS04	MN	B	E103 N149 L150 D188	E102 N148 L149 D187

Gene Ontology

Molecular Function

• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

External links

• PDB: RCSB:3dw9, PDBe:3dw9, PDBj:3dw9
• PDBsum: 3dw9
• PubMed: 18701646
• UniProt: Q9F6L0