Structure of PDB 3du4 Chain B

Receptor sequence

>3du4B (length=448) Species: 1423 (Bacillus subtilis)

MTHDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVKDINGKEYYDGF
SSVWLNVHGHRKKELDDAIKKQLGKIAHSTLLGMTNVPATQLAETLIDIS
PKKLTRVFYSDSGAEAMEIALKMAFQYWKNIGKPEKQKFIAMKNGYHGDT
IGAVSVGSIELFHHVYGPLMFESYKAPIPYVYRSESGDPDECRDQCLREL
AQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVRELCTTYDVLMIV
DEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYK
AFYDDYENLKTFFHGHSYTGNQLGCAVALENLALFESENIVEQVAEKSKK
LHFLLQDLHALPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSL
KMRELGMLTRPLGDVIAFLPPLASTAEELSEMVAIMKQAIHEVTSLED

3D structure

• PDB: 3du4 Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): F17 Y146 E217 D251 A254 K280 A417
• Catalytic site (residue number reindexed from 1): F17 Y146 E217 D251 A254 K280 A417
• Enzyme Commision number: 2.6.1.105: lysine--8-amino-7-oxononanoate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	B	H316 S317	H316 S317
BS02	KAP	B	L82 G315	L82 G315
BS03	PLP	B	G113 A114 Y146 H147 E217 D251 V253 K280	G113 A114 Y146 H147 E217 D251 V253 K280
BS04	KAP	B	Y146 R410	Y146 R410

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0009102 biotin biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3du4, PDBe:3du4, PDBj:3du4
• PDBsum: 3du4
• PubMed: 20565114
• UniProt: P53555|BIOK_BACSU L-Lysine--8-amino-7-oxononanoate transaminase (Gene Name=bioK)