Structure of PDB 3dla Chain B

Receptor sequence

>3dlaB (length=649) Species: 1773 (Mycobacterium tuberculosis)

MNFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVF
PELTLSGYSIEDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHR
HRIYNTAVVIHRGAVLGVVPKSYLPTYREFYERRQMAPGDGERGTIRIGG
ADVAFGTDLLFAASDLPGFVLHVEICEDMFVPMPPSAEAALAGATVLANL
SGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTMI
WENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELT
ESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSG
LEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALP
GKNNAIKLARALGVTFSEIDIGDTARLMLHTIGHVTFENVQAGLRTDYLF
RIANQRGGIVLGTGDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLI
RWVISAGEFGEKVGEVLQSVLDTEITSEAKVGPFALQDFSLFQVLRYGFR
PSKIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQIFVQRFYS
FSQFKRSALPNGPKVSHGGALSPRGDWRAPSDMSARIWLDQIDREVPKG

3D structure

• PDB: 3dla Regulation of active site coupling in glutamine-dependent NAD(+) synthetase.
• Chain: B
• Resolution: 2.35 Å

Enzymatic activity

• Enzyme Commision number: 6.3.5.1: NAD(+) synthase (glutamine-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ONL	B	C176 F180 S201 S203 R209 Y230	C176 F180 S201 S203 R209 Y230
BS02	DND	B	W490 S491 T492 D497 F631 F634 K635	W473 S474 T475 D480 F601 F604 K605
BS03	DND	B	L358 G475 H501	L358 G458 H484

Gene Ontology

Molecular Function

• GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity
• GO:0004359 glutaminase activity
• GO:0005524 ATP binding
• GO:0008795 NAD+ synthase activity
• GO:0016874 ligase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0009435 NAD biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:3dla, PDBe:3dla, PDBj:3dla
• PDBsum: 3dla
• PubMed: 19270703
• UniProt: P9WJJ3|NADE_MYCTU Glutamine-dependent NAD(+) synthetase (Gene Name=nadE)