Structure of PDB 3d9f Chain B

Receptor sequence

>3d9fB (length=431) Species: 5507 (Fusarium oxysporum)

VDFKLSPSQLEARRHAQAFANTVLTKASAEYSTQKDQLSRFQATRPFYRE
AVRHGLIKAQVPIPLGGTMESLVHESIILEELFAVEPATSITIVATALGL
MPVILCDSPSLQEKFLKPFISGEGEPLASLMHSEPNGTANWLQKGGPGLQ
TTARKVGNEWVISGEKLWPSNSGGWDYKGADLACVVCRVSDDPSKPQDPN
VDPATQIAVLLVTRETIANNKKDAYQILGEPELAGHITTSGPHTRFTEFH
VPHENLLCTPGLKAQGLVETAFAMAAALVGAMAIGTARAAFEEALVFAKS
DTRGGSKHIIEHQSVADKLIDCKIRLETSRLLVWKAVTTLEDEALEWKVK
LEMAMQTKIYTTDVAVECVIDAMKAVGMKSYAKDMSFPRLLNEVMCYPLF
DGGNIGLRRRQMQRVMALEDYEPWAATYGSS

3D structure

• PDB: 3d9f Crystal structures of intermediates in the nitroalkane oxidase reaction.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H133 S134 D402
• Catalytic site (residue number reindexed from 1): H132 S133 D401
• Enzyme Commision number: 1.7.3.1: nitroalkane oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	R304 I310 H313 V316 K375 A376 V377 G378 M379	R303 I309 H312 V315 K374 A375 V376 G377 M378
BS02	FAD	B	L131 H133 S134 G138 T139 A140 N141 W169 P170 F401 D402 G403 G404 I406 G407	L130 H132 S133 G137 T138 A139 N140 W168 P169 F400 D401 G402 G403 I405 G406
BS03	N6C	B	V95 L99 F401 D402	V94 L98 F400 D401	MOAD: Ki=130mM

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003995 acyl-CoA dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
• GO:0050660 flavin adenine dinucleotide binding
• GO:0052664 nitroalkane oxidase activity
• GO:0071949 FAD binding

Biological Process

• GO:0033539 fatty acid beta-oxidation using acyl-CoA dehydrogenase
• GO:0046359 butyrate catabolic process
• GO:0098754 detoxification

External links

• PDB: RCSB:3d9f, PDBe:3d9f, PDBj:3d9f
• PDBsum: 3d9f
• PubMed: 19265437
• UniProt: Q8X1D8|NAO_FUSOX Nitroalkane oxidase